{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:07:13Z","timestamp":1791295633503,"version":"4.1.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2001,3,1]],"date-time":"2001-03-01T00:00:00Z","timestamp":983404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Listeria monocytogenes is an intracellular bacterium that causes systemic infections after traversing the intestinal mucosa. Clearance of infection and long term protective immunity are mediated by L. monocytogenes-specific CD8 T lymphocytes. In this report, we characterize the murine CD8 T cell response in the lamina propria and intestinal epithelium after enteric L. monocytogenes infection. We find that the frequency of MHC class Ia-restricted, L. monocytogenes-specific T cells is \u223c4- to 5-fold greater in the lamina propria than in the spleen of mice after oral or i.v. infection. Although the kinetics of T cell expansion and contraction are similar in spleen, lamina propria, and intestinal epithelium, high frequencies of Ag-specific T cells are detected only in the lamina propria 1 mo after infection. In contrast to MHC class Ia-restricted T cells, the frequency of H2-M3-restricted, L. monocytogenes-specific T cells is decreased in the intestinal mucosa relative to that found in the spleen. In addition to this disparity, we find that MHC class Ia-restricted CD8 T cells specific for a dominant L. monocytogenes epitope have different TCR V\u03b2 repertoires in the spleen and intestinal mucosa of individual mice. These findings indicate that the intestinal mucosa is a depot where L. monocytogenes-specific effector CD8 T cells accumulate during and after infection irrespective of immunization route. Furthermore, our results demonstrate that CD8 T cell populations in these two sites, although overlapping in Ag specificity, are distinct in terms of their repertoire.<\/jats:p>","DOI":"10.4049\/jimmunol.166.6.4065","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T23:32:32Z","timestamp":1398036752000},"page":"4065-4073","source":"Crossref","is-referenced-by-count":56,"title":["Intestinal and Splenic T Cell Responses to Enteric <i>Listeria monocytogenes<\/i> Infection: Distinct Repertoires of Responding CD8 T Lymphocytes"],"prefix":"10.1093","volume":"166","author":[{"given":"James W","family":"Huleatt","sequence":"first","affiliation":[{"name":"Sections of Infectious Diseases and Immunobiology, Yale School of Medicine , New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Pilip","sequence":"additional","affiliation":[{"name":"Sections of Infectious Diseases and Immunobiology, Yale School of Medicine , New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen","family":"Kerksiek","sequence":"additional","affiliation":[{"name":"Sections of Infectious Diseases and Immunobiology, Yale School of Medicine , New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric G","family":"Pamer","sequence":"additional","affiliation":[{"name":"Sections of Infectious Diseases and Immunobiology, Yale School of Medicine , New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,3,15]]},"reference":[{"key":"2025032714005893100_R1","first-page":"1313","volume-title":"JAMA","year":"1989"},{"key":"2025032714005893100_R2","first-page":"392","volume-title":"Am. 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