{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:51:35Z","timestamp":1787730695619,"version":"build-2784847793"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>CD100 is the first semaphorin described in lymphoid tissues, where it has been shown to be associated with a serine kinase activity. Semaphorins are molecules involved in axon pathfinding during nerve development and act as repellent guidance cues. In the nervous system semaphorins exist as either membrane-bound or secreted forms. We report here a spontaneous processing of membrane CD100, suggesting that it is also produced as a diffusable semaphorin from lymphoid cells. Monomeric and homodimeric forms of CD100 are expressed by T lymphocytes and CD100-transfected fibroblasts. We demonstrate that CD100 is released through a proteolytic process blocked by metalloprotease inhibitors. In T cells, only soluble CD100 dimers are produced, suggesting that CD100 dimerization is required for proteolysis. In agreement, we observe that increasing membrane dimers strongly favors shedding of the molecule. By expressing a CD100 molecule mutated at cysteine 674 into a COS cell system, we additionally demonstrate that this particular residue in the extracellular domain of the molecule is required for dimerization. Finally, we show that staurosporine, a serine kinase inhibitor, enhances the membrane cleavage of CD100. Together these results demonstrate that membrane CD100 is cleaved by a metalloprotease-dependent process, which is probably regulated by phosphorylation. Mainly, these findings shed light on a possible function for the semaphorin region of CD100 as a long range guidance cue in the immune system.<\/jats:p>","DOI":"10.4049\/jimmunol.166.7.4341","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:32:21Z","timestamp":1398025941000},"page":"4341-4347","source":"Crossref","is-referenced-by-count":115,"title":["Biological Activity of Soluble CD100. I. The Extracellular Region of CD100 Is Released from the Surface of T Lymphocytes by Regulated Proteolysis"],"prefix":"10.1093","volume":"166","author":[{"given":"Abdellah","family":"Elhabazi","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 448, Faculte\u0301 de Me\u0301decine de Cre\u0301teil , Cre\u0301teil ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phanie","family":"Delaire","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 448, Faculte\u0301 de Me\u0301decine de Cre\u0301teil , Cre\u0301teil ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armand","family":"Bensussan","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 448, Faculte\u0301 de Me\u0301decine de Cre\u0301teil , Cre\u0301teil ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Boumsell","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 448, Faculte\u0301 de Me\u0301decine de Cre\u0301teil , Cre\u0301teil ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Bismuth","sequence":"additional","affiliation":[{"name":"Laboratoire d\u2019Immunologie Cellulaire, Centre National de la Recherche Scientifique, Unite\u0301 Mixte de Recherche 7627, Centre Hospitalier Pitie\u0301-Salpe\u0302trie\u0300re , Paris ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,4,1]]},"reference":[{"key":"2026020208021960200_R1","first-page":"318","volume-title":"J. 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