{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:30:12Z","timestamp":1770910212667,"version":"3.50.1"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To evaluate the role of CCR2 in allergic asthma, mutant mice deficient in CCR2 (CCR2\u2212\/\u2212) and intact mice were sensitized with i.p. OVA with alum on days 0 and 7, and challenged by inhalation with nebulization of either OVA or saline. Airway hyperreactivity, measured by the methacholine-provoked increase in enhanced pause, was significantly increased (p &amp;lt; 0.05) in OVA-challenged CCR2\u2212\/\u2212 mutant mice, compared with comparably challenged CCR2+\/+ mice. OVA-challenged CCR2\u2212\/\u2212 mutants also were also found to have enhanced bronchoalveolar lavage fluid eosinophilia, peribronchiolar cellular cuffing, and Ig subclass switching, with increase in OVA-specific IgG1 and IgE. In addition, RNase protection assay revealed increased whole lung expression of IL-13 in OVA-challenged CCR2\u2212\/\u2212 mutants. Unexpectedly, serum monocyte chemotactic protein-1 levels were 8-fold higher in CCR2\u2212\/\u2212 mutants than in CCR2+\/+ mice sensitized to OVA, but OVA challenge had no additional effect on circulating monocyte chemotactic protein-1 in either genotype. Ag stimulation of lymphocytes isolated from OVA-sensitized CCR2 mutants revealed a significant increase (p &amp;lt; 0.05) in IL-5 production, which differed from OVA-stimulated lymphocytes from sensitized CCR2+\/+ mice. These experiments demonstrate an enhanced response in airway reactivity and in lung inflammation in CCR2\u2212\/\u2212 mutant mice compared with comparably sensitized and challenged CCR2+\/+ mice. These observations suggest that CC chemokines and their receptors are involved in immunomodulation of atopic asthma.<\/jats:p>","DOI":"10.4049\/jimmunol.166.8.5183","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:57:27Z","timestamp":1398031047000},"page":"5183-5192","source":"Crossref","is-referenced-by-count":60,"title":["Enhanced Airway Th2 Response After Allergen Challenge in Mice Deficient in CC Chemokine Receptor-2 (CCR2)"],"prefix":"10.1093","volume":"166","author":[{"given":"YongBok","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, University of Virginia Health System , Charlottesville, VA 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-sang J","family":"Sung","sequence":"additional","affiliation":[{"name":"Division of Rheumatology and Immunology, University of Virginia Health System , Charlottesville, VA 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William A","family":"Kuziel","sequence":"additional","affiliation":[{"name":"Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas , Austin, TX 78712"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanford","family":"Feldman","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine, University of Virginia Health System , Charlottesville, VA 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu Man","family":"Fu","sequence":"additional","affiliation":[{"name":"Division of Rheumatology and Immunology, University of Virginia Health System , Charlottesville, VA 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"C Edward","family":"Rose","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, University of Virginia Health System , Charlottesville, VA 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,4,15]]},"reference":[{"key":"2026020209181819900_R1","first-page":"138","volume-title":"Immunol. 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