{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:48:05Z","timestamp":1790776085777,"version":"4.1.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2001,7,1]],"date-time":"2001-07-01T00:00:00Z","timestamp":993945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In the present study, we have investigated the possibility to engage the Yersinia outer protein E (YopE) as a carrier molecule for heterologous Ag delivery by the type III secretion system of Salmonella typhimurium. Defined secretion and translocation domains of YopE were fused to the immunodominant T cell Ags listeriolysin O and p60 of Listeria monocytogenes. In vitro experiments showed that S. typhimurium allows secretion and translocation of large hybrid YopE proteins in a type III-dependent fashion. Translocation and cytosolic delivery of these chimeric proteins into host cells, but not secretion into endosomal compartments, led to efficient MHC class I-restricted Ag presentation of listerial nonamer peptides. Mice orally vaccinated with a single dose of attenuated S. typhimurium expressing translocated hybrid YopE proteins revealed high numbers of IFN-\u03b3-producing cells reactive with listeriolysin O 91\u201399 or p60 217\u2013225, respectively. This CD8 T cell response protected mice against a challenge with L. monocytogenes. In conclusion, these findings suggest that YopE is a versatile carrier molecule for type III-mediated foreign Ag delivery by Salmonella vaccine strains.<\/jats:p>","DOI":"10.4049\/jimmunol.167.1.357","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:28:05Z","timestamp":1398025685000},"page":"357-365","source":"Crossref","is-referenced-by-count":72,"title":["Protection Against Murine Listeriosis by Oral Vaccination with Recombinant\n                    <i>Salmonella<\/i>\n                    Expressing Hybrid\n                    <i>Yersinia<\/i>\n                    Type III Proteins"],"prefix":"10.1093","volume":"167","author":[{"given":"Holger","family":"Ru\u0308ssmann","sequence":"first","affiliation":[{"name":"Max von Pettenkofer-Institut fu\u0308r Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universita\u0308t Mu\u0308nchen , Mu\u0308nchen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emeka I","family":"Igwe","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer-Institut fu\u0308r Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universita\u0308t Mu\u0308nchen , Mu\u0308nchen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeannette","family":"Sauer","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer-Institut fu\u0308r Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universita\u0308t Mu\u0308nchen , Mu\u0308nchen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolf-Dietrich","family":"Hardt","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer-Institut fu\u0308r Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universita\u0308t Mu\u0308nchen , Mu\u0308nchen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Bubert","sequence":"additional","affiliation":[{"name":"Lehrstuhl fu\u0308r Mikrobiologie, Theodor-Boveri-Institut fu\u0308r Biowissenschaften , Am Hubland, Wu\u0308rzburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gernot","family":"Geginat","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Hygiene, Fakulta\u0308t fu\u0308r Klinische Medizin Mannheim der Universita\u0308t Heidelberg , Mannheim ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,7,1]]},"reference":[{"key":"2026020208041446100_R1","first-page":"23","volume-title":"Dev. 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