{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:20:01Z","timestamp":1792120801025,"version":"4.4.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2001,11,1]],"date-time":"2001-11-01T00:00:00Z","timestamp":1004572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Although the role of CD28-B7 interaction in the activation of naive T cells is well established, its importance in the generation and maintenance of T cell memory is not well understood. In this study, we examined the requirement for CD28-B7 interactions in primary T cell activation and immune memory. Ag-specific CD8 T cell responses were compared between wild-type (+\/+) and CD28-deficient (CD28\u2212\/\u2212) mice following an acute infection with lymphocytic choriomeningitis virus (LCMV). During the primary response, there was a substantial activation and expansion of LCMV-specific CD8 T cells in both +\/+ and CD28\u2212\/\u2212 mice. However, the magnitude of the primary CD8 T cell response to both dominant and subdominant LCMV CTL epitopes was \u223c2- to 3-fold lower in CD28\u2212\/\u2212 mice compared with +\/+ mice; the lack of CD28-mediated costimulation did not lead to preferential suppression of CD8 T cell responses to the weaker subdominant epitopes. As seen in CD28\u2212\/\u2212 mice, blockade of B7-mediated costimulation by CTLA4-Ig treatment of +\/+ mice also resulted in a 2-fold reduction in the anti-LCMV CD8 T cell responses. Loss of CD28\/B7 interactions did not significantly affect the generation and maintenance of CD8 T cell memory; the magnitude of CD8 T cell memory was \u223c2-fold lower in CD28\u2212\/\u2212 mice as compared with +\/+ mice. Further, in CD28\u2212\/\u2212 mice, LCMV-specific memory CD8 T cells showed normal homeostatic proliferation in vivo and also conferred protective immunity. Therefore, CD28 signaling is not necessary for the proliferative renewal and maintenance of memory CD8 T cells.<\/jats:p>","DOI":"10.4049\/jimmunol.167.10.5565","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:55:17Z","timestamp":1398034517000},"page":"5565-5573","source":"Crossref","is-referenced-by-count":151,"title":["Role of CD28-B7 Interactions in Generation and Maintenance of CD8 T Cell Memory"],"prefix":"10.1093","volume":"167","author":[{"given":"M","family":"Suresh","sequence":"first","affiliation":[{"name":"Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine , Atlanta, GA 30322"},{"name":"Department of Pathobiological Sciences, University of Wisconsin , Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason K","family":"Whitmire","sequence":"additional","affiliation":[{"name":"Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie E","family":"Harrington","sequence":"additional","affiliation":[{"name":"Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian P","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Surgery, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C","family":"Pearson","sequence":"additional","affiliation":[{"name":"Department of Surgery, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D","family":"Altman","sequence":"additional","affiliation":[{"name":"Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafi","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine , Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,11,15]]},"reference":[{"key":"2026020208054386600_R1","first-page":"501","volume-title":"Immunity","year":"1994"},{"key":"2026020208054386600_R2","first-page":"423","volume-title":"Immunol. 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