{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:52:18Z","timestamp":1791046338023,"version":"4.1.0"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2001,11,1]],"date-time":"2001-11-01T00:00:00Z","timestamp":1004572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infection of mice with the intracellular bacterium Listeria monocytogenes results in a strong CD8+ T cell response that is critical for efficient control of infection. We used CD28-deficient mice to characterize the function of CD28 during Listeria infection, with a main emphasis on Listeria-specific CD8+ T cells. Frequencies and effector functions of these T cells were determined using MHC class I tetramers, single cell IFN-\u03b3 production and Listeria-specific cytotoxicity. During primary Listeria infection of CD28\u2212\/\u2212 mice we observed significantly reduced numbers of Listeria-specific CD8+ T cells and only marginal levels of specific IFN-\u03b3 production and cytotoxicity. Although frequencies were also reduced in CD28\u2212\/\u2212 mice during secondary response, we detected a considerable population of Listeria-specific CD8+ T cells in these mice. In parallel, IFN-\u03b3 production and cytotoxicity were observed, revealing that Listeria-specific CD8+ T cells in CD28\u2212\/\u2212 mice expressed normal effector functions. Consistent with their impaired CD8+ T cell activation, CD28\u2212\/\u2212 mice suffered from exacerbated listeriosis both after primary and secondary infection. These results demonstrate participation of CD28 signaling in the generation and expansion of Ag-specific CD8+ T cells in listeriosis. However, Ag-specific CD8+ T cells generated in the absence of CD28 differentiated into normal effector and memory T cells.<\/jats:p>","DOI":"10.4049\/jimmunol.167.10.5620","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:55:17Z","timestamp":1398034517000},"page":"5620-5627","source":"Crossref","is-referenced-by-count":64,"title":["Role of CD28 for the Generation and Expansion of Antigen-Specific CD8+ T Lymphocytes During Infection with\n                    <i>Listeria monocytogenes<\/i>"],"prefix":"10.1093","volume":"167","author":[{"given":"Hans-Willi","family":"Mittru\u0308cker","sequence":"first","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology , Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mischo","family":"Kursar","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology , Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Ko\u0308hler","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology , Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Hurwitz","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology , Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan H E","family":"Kaufmann","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Planck Institute for Infection Biology , Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,11,15]]},"reference":[{"key":"2026020208044484900_R1","first-page":"381","volume-title":"J. 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