{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:37:04Z","timestamp":1770079024801,"version":"3.49.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>H2-DM (DM, previously H2-M) facilitates the exchange of peptides bound to MHC class II molecules. In this study, we have used H2-DM-deficient (DM\u2212\/\u2212) mice to analyze the influence of DM in the priming of B cell responses in vivo and for Ag presentation by B cells in vitro. After immunization, IgG Abs could be raised to a T-dependent Ag, 4-hydroxy-5-nitrophenylacetyl-OVA, in DM\u2212\/\u2212 mice, but closer analysis revealed the IgG response to be slower, diminished in titer, and composed of low-affinity Abs. The Ab response correlated with a vast reduction in the number of germinal centers in the spleen. The presentation of multiple epitopes by H2-Ab from distinct Ags was found to be almost exclusively DM-dependent whether B cells internalized Ags via fluid phase uptake or using membrane Ig receptors. The poor B cell response in vivo could be largely, but not completely restored by expression of a H2-Ead transgene, despite the fact that Ag presentation by H2-Ed\/b molecules was found to be highly DM dependent. Hence, while substantial Ab responses can be raised in the absence of DM, this molecule is a crucial factor both for Ag processing and for the normal maturation of T-dependent humoral immune responses in vivo.<\/jats:p>","DOI":"10.4049\/jimmunol.167.11.6348","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:08:34Z","timestamp":1398024514000},"page":"6348-6355","source":"Crossref","is-referenced-by-count":25,"title":["The Impact of H2-DM on Humoral Immune Responses"],"prefix":"10.1093","volume":"167","author":[{"given":"Christopher","family":"Alfonso","sequence":"first","affiliation":[{"name":"R. W. Johnson Pharmaceutical Research Institute , San Diego, CA 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung-Ok","family":"Han","sequence":"additional","affiliation":[{"name":"R. W. 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