{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:09:05Z","timestamp":1790395745818,"version":"4.1.0"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The adherence of Plasmodium falciparum-infected RBC (IRBC) to postcapillary venular endothelium is an important determinant of the pathogenesis of severe malaria complications. Cytoadherence of IRBC to endothelial cells involves specific receptor\/ligand interactions. The glycoprotein CD36 expressed on endothelial cells is the major receptor involved in this interaction. Treatment of CD36-expressing cells with reducing agents, such as DTT and N-acetylcysteine, was followed by CD36 conformational change monitorable by the appearance of the Mo91 mAb epitope. Only a fraction of the surface expressed CD36 molecules became Mo91 positive, suggesting the presence of two subpopulations of molecules with different sensitivities to reduction. The Mo91 epitope has been localized on a peptide (residues 260\u2013279) of the C-terminal, cysteine-rich region of CD36. Treatment with reducing agents inhibited the CD36-dependent cytoadherence of IRBC to CD36-expressing cells and dissolved pre-existent CD36-mediated IRBC\/CD36-expressing cell aggregates. CD36 reduction did not impair the functionality of CD36, since the reactivity of other anti-CD36 mAbs as well as the binding of oxidized low density lipoprotein, a CD36 ligand, were maintained. The modifications induced by reduction were reversible. After 14 h CD36 was reoxidized, the cells did not express the Mo91 epitope, and cytoadherence to IRBC was restored. The results indicate that IRBCs bind only to a redox-modulated fraction of CD36 molecules expressed on the cell surface. The present data indicate the therapeutic potential of reducing agents, such as the nontoxic drug N-acetylcysteine, to prevent or treat malaria complications due to IRBC cytoadhesion.<\/jats:p>","DOI":"10.4049\/jimmunol.167.11.6510","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:08:34Z","timestamp":1398024514000},"page":"6510-6517","source":"Crossref","is-referenced-by-count":13,"title":["Cytoadherence of\n                    <i>Plasmodium falciparum<\/i>\n                    -Infected Erythrocytes Is Mediated by a Redox-Dependent Conformational Fraction of CD36"],"prefix":"10.1093","volume":"167","author":[{"given":"Paola","family":"Gruarin","sequence":"first","affiliation":[{"name":"DIBIT, San Raffaele Scientific Institute , Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Primo","sequence":"additional","affiliation":[{"name":"Molecular Angiogenesis Unit, Institute for Cancer Research and Treatment , Candiolo ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Ferrandi","sequence":"additional","affiliation":[{"name":"Molecular Angiogenesis Unit, Institute for Cancer Research and Treatment , Candiolo ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Bussolino","sequence":"additional","affiliation":[{"name":"Molecular Angiogenesis Unit, Institute for Cancer Research and Treatment , Candiolo ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narendra N","family":"Tandon","sequence":"additional","affiliation":[{"name":"Otsuka America Pharmaceutical, Inc., Maryland Research Laboratories , Rockville, MD 20850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Arese","sequence":"additional","affiliation":[{"name":"Department of Genetics, Biology, and Biochemistry, University of Torino Medical School , Torino ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Ulliers","sequence":"additional","affiliation":[{"name":"Department of Genetics, Biology, and Biochemistry, University of Torino Medical School , Torino ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Alessio","sequence":"additional","affiliation":[{"name":"DIBIT, San Raffaele Scientific Institute , Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,12,1]]},"reference":[{"key":"2026020208145192600_R1","volume-title":"The world health report","year":"1998"},{"key":"2026020208145192600_R2","first-page":"C1231","volume-title":"Am. J. Physiol.","year":"1999"},{"key":"2026020208145192600_R3","first-page":"3766","volume-title":"Blood","year":"1997"},{"key":"2026020208145192600_R4","first-page":"77","volume-title":"Cell","year":"1995"},{"key":"2026020208145192600_R5","first-page":"416","volume-title":"Parasitol. Today","year":"2000"},{"key":"2026020208145192600_R6","first-page":"2292","volume-title":"Blood","year":"2000"},{"key":"2026020208145192600_R7","first-page":"95","volume-title":"Cell","year":"1989"},{"key":"2026020208145192600_R8","first-page":"689","volume-title":"Nature","year":"1992"},{"key":"2026020208145192600_R9","first-page":"287","volume-title":"Cell","year":"1997"},{"key":"2026020208145192600_R10","first-page":"1469","volume-title":"Science","year":"1989"},{"key":"2026020208145192600_R11","first-page":"1105","volume-title":"Blood","year":"1992"},{"key":"2026020208145192600_R12","first-page":"3637","volume-title":"Blood","year":"1993"},{"key":"2026020208145192600_R13","first-page":"1436","volume-title":"Science","year":"1993"},{"key":"2026020208145192600_R14","first-page":"2121","volume-title":"Blood","year":"1999"},{"key":"2026020208145192600_R15","first-page":"1205","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026020208145192600_R16","first-page":"349","volume-title":"Parasitol. Immunol.","year":"2000"},{"key":"2026020208145192600_R17","first-page":"446","volume-title":"Biochem. Biophys. Acta","year":"2000"},{"key":"2026020208145192600_R18","first-page":"635","volume-title":"Biochem. J.","year":"1997"},{"key":"2026020208145192600_R19","first-page":"473","volume-title":"J. Infect. Dis.","year":"1999"},{"key":"2026020208145192600_R20","first-page":"487","volume-title":"Cell. Immunol.","year":"1991"},{"key":"2026020208145192600_R21","first-page":"7570","volume-title":"J. Biol. Chem.","year":"1989"},{"key":"2026020208145192600_R22","first-page":"2229","volume-title":"Infect. Immun.","year":"1993"},{"key":"2026020208145192600_R23","first-page":"1770","volume-title":"J. Biol. Chem.","year":"1996"},{"key":"2026020208145192600_R24","first-page":"3587","volume-title":"Nucleic Acids Res.","year":"1990"},{"key":"2026020208145192600_R25","first-page":"8392","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1993"},{"key":"2026020208145192600_R26","first-page":"2745","volume-title":"J. Clin. Invest.","year":"1973"},{"key":"2026020208145192600_R27","first-page":"418","volume-title":"J. Parasitol.","year":"1979"},{"key":"2026020208145192600_R28","first-page":"1601","volume-title":"Infect. Immun.","year":"1998"},{"key":"2026020208145192600_R29","first-page":"740","volume-title":"Br. J. Haematol.","year":"1994"},{"key":"2026020208145192600_R30","first-page":"89","volume-title":"Mol. Cell. Biochem","year":"2000"},{"key":"2026020208145192600_R31","first-page":"707","volume-title":"J. Cell Biol.","year":"1997"},{"key":"2026020208145192600_R32","first-page":"1814","volume-title":"Blood","year":"1998"},{"key":"2026020208145192600_R33","first-page":"812","volume-title":"Biochem. Biophys. Acta","year":"1997"},{"key":"2026020208145192600_R34","first-page":"897","volume-title":"Thromb. Haemost.","year":"1997"},{"key":"2026020208145192600_R35","first-page":"111","volume-title":"J. Cell Biol.","year":"1994"},{"key":"2026020208145192600_R36","first-page":"67","volume-title":"Biochem. J.","year":"1996"},{"key":"2026020208145192600_R37","first-page":"5501","volume-title":"EMBO J.","year":"1997"},{"key":"2026020208145192600_R38","first-page":"3766","volume-title":"Blood","year":"1997"},{"key":"2026020208145192600_R39","first-page":"1311","volume-title":"Mol. Biol. Cell","year":"1994"},{"key":"2026020208145192600_R40","first-page":"2569","volume-title":"J. Immunol.","year":"1999"},{"key":"2026020208145192600_R41","first-page":"330","volume-title":"Brain Res.","year":"1999"},{"key":"2026020208145192600_R42","first-page":"8853","volume-title":"Biochemistry","year":"1999"},{"key":"2026020208145192600_R43","first-page":"73","volume-title":"Nature","year":"1999"},{"key":"2026020208145192600_R44","first-page":"8750","volume-title":"Proc. Natl. Acad. Sci. USA","year":"2001"},{"key":"2026020208145192600_R45","first-page":"77","volume-title":"Eur. J. Clin. Pharmacol.","year":"1988"},{"key":"2026020208145192600_R46","first-page":"31","volume-title":"Respiration","year":"1986"},{"key":"2026020208145192600_R47","first-page":"1852","volume-title":"N. Engl. J. Med.","year":"1991"},{"key":"2026020208145192600_R48","first-page":"1557","volume-title":"N. Engl. J. Med.","year":"1988"},{"key":"2026020208145192600_R49","first-page":"498","volume-title":"J. Acquir. Immune Defic. Syndr. Hum. Retrovirol.","year":"1998"},{"key":"2026020208145192600_R50","first-page":"141","volume-title":"AIDS Res. Hum. Retroviruses","year":"1995"},{"key":"2026020208145192600_R51","first-page":"1589","volume-title":"AIDS Res. Hum. Retroviruses","year":"1998"},{"key":"2026020208145192600_R52","first-page":"1026","volume-title":"Br. Med. J.","year":"1991"},{"key":"2026020208145192600_R53","first-page":"1","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026020208145192600_R54","first-page":"2664","volume-title":"Proc. Natl. Acad. Sci. USA","year":"2001"},{"key":"2026020208145192600_R55","first-page":"389","volume-title":"Am. J. Trop. Med. Hyg.","year":"1997"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/167\/11\/6510\/62544549\/6510.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/167\/11\/6510\/62544549\/6510.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T13:25:43Z","timestamp":1770038743000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/167\/11\/6510\/8017600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12,1]]},"references-count":55,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2001,12,1]]},"published-print":{"date-parts":[[2001,12,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.167.11.6510","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2001,12]]},"published":{"date-parts":[[2001,12,1]]}}}