{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:24:21Z","timestamp":1791037461423,"version":"4.1.0"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2001,7,1]],"date-time":"2001-07-01T00:00:00Z","timestamp":993945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The transcription factor GATA-3 is essential for early T cell development and differentiation of naive CD4+ T cells into Th2 effector cells. To study the function of GATA-3 during T cell-mediated immune responses in vivo, we investigated CD2-GATA3-transgenic mice in which GATA-3 expression is driven by the CD2 locus control region. Both in the CD4+ and the CD8+ T cell population the proportion of cells exhibiting a CD44highCD45RBlowCD62Llow Ag-experienced phenotype was increased. In CD2-GATA3-transgenic mice, large fractions of peripheral CD4+ T cells expressed the IL-1 receptor family member T1\/ST2, indicative of advanced Th2 commitment. Upon in vitro T cell stimulation, the ability to produce IL-2 and IFN-\u03b3 was decreased. Moreover, CD4+ T cells manifested rapid secretion of the Th2 cytokines IL-4, IL-5, and IL-10, reminiscent of Th2 memory cells. In contrast to wild-type CD4+ cells, which lost GATA-3 expression when cultured under Th1-polarizing conditions, CD2-GATA3-transgenic CD4+ cells maintained expression of GATA-3 protein. Under Th1 conditions, cellular proliferation of CD2-GATA3-transgenic CD4+ cells was severely hampered, IFN-\u03b3 production was decreased and Th2 cytokine production was increased. Enforced GATA-3 expression inhibited Th1-mediated in vivo responses, such as Ag-specific IgG2a production or a delayed-type hypersensitivity response to keyhole limpet hemocyanin. Collectively, these observations indicate that enforced GATA-3 expression selectively inhibits Th1 differentiation and induces Th2 differentiation. The increased functional capacity to secrete Th2 cytokines, along with the increased expression of surface markers for Ag-experienced Th2-committed cells, would argue for a role of GATA-3 in Th2 memory formation.<\/jats:p>","DOI":"10.4049\/jimmunol.167.2.724","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T22:17:55Z","timestamp":1398118675000},"page":"724-732","source":"Crossref","is-referenced-by-count":77,"title":["Enforced Expression of GATA-3 in Transgenic Mice Inhibits Th1 Differentiation and Induces the Formation of a T1\/ST2-Expressing Th2-Committed T Cell Compartment In Vivo"],"prefix":"10.1093","volume":"167","author":[{"given":"Martijn C","family":"Nawijn","sequence":"first","affiliation":[{"name":"Department of Immunology, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma M","family":"Dingjan","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]},{"name":"Department of Cell Biology and Genetics, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart N","family":"Lambrecht","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, Faculty of Medicine, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alar","family":"Karis","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]},{"name":"Institute of Molecular and Cell Biology, University of Tartu , Tartu ,","place":["Estonia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Grosveld","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huub","family":"Savelkoul","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf W","family":"Hendriks","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]},{"name":"Department of Cell Biology and Genetics, Erasmus University Rotterdam , Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,7,15]]},"reference":[{"key":"2026020209095974700_R1","first-page":"2348","volume-title":"J. 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