{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:56:09Z","timestamp":1791158169675,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2001,7,1]],"date-time":"2001-07-01T00:00:00Z","timestamp":993945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We have previously reported that B cell receptors, depending on the degree to which they are cross-linked, can promote apoptosis in various human B cell types. In this study, we show that B cell receptors can trigger two apoptotic pathways according to cross-linking and that these pathways control mitochondrial activation in human Burkitt\u2019s lymphoma cells. Whereas soluble anti-\u03bc Ab triggers caspase-independent mitochondrial activation, cross-linked anti-\u03bc Ab induces an apoptotic response associated with a caspase-dependent loss of mitochondrial transmembrane potential. This B cell receptor-mediated caspase-dependent mitochondrial activation is associated with caspase-8 activation. We show here that caspase-8 inhibitors strongly decrease cross-linking-dependent B cell receptor-mediated apoptosis in Burkitt\u2019s lymphoma BL41 cells. These inhibitors act upstream from the mitochondria as they prevented the loss of mitochondrial membrane potential observed in B cell receptor-treated BL41 cells. Caspase-8 activation in these cells was also evident from the detection of cleaved fragments of caspase-8 and the cleavage of specific substrates, including Bid. Our data show that cross-linked B cell receptors induced an apoptotic pathway involving sequential caspase-8 activation, loss of mitochondrial membrane potential, and the activation of caspase-9 and caspase-3. Cells expressing a dominant negative mutant of Fas-associated death domain protein were sensitive to cross-linked B cell receptor-induced caspase-8 activation and apoptosis; therefore, this caspase-8 activation was independent of the death effector domain of Fas-associated death domain protein.<\/jats:p>","DOI":"10.4049\/jimmunol.167.2.733","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T22:17:55Z","timestamp":1398118675000},"page":"733-740","source":"Crossref","is-referenced-by-count":36,"title":["B Cell Receptor Cross-Linking Triggers a Caspase-8- Dependent Apoptotic Pathway That Is Independent of the Death Effector Domain of Fas-Associated Death Domain Protein"],"prefix":"10.1093","volume":"167","author":[{"given":"Laurence","family":"Besnault","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 131 and Unite\u0301 542, and Claude Bernard Research Center , Clamart ,","place":["France"]},{"name":"Ho\u0302pital Paul Brousse , Villejuif ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Schrantz","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 131 and Unite\u0301 542, and Claude Bernard Research Center , Clamart ,","place":["France"]},{"name":"Ho\u0302pital Paul Brousse , Villejuif ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie The\u0301re\u0300se","family":"Auffredou","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 131 and Unite\u0301 542, and Claude Bernard Research Center , Clamart ,","place":["France"]},{"name":"Ho\u0302pital Paul Brousse , Villejuif ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge\u0301rald","family":"Leca","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 131 and Unite\u0301 542, and Claude Bernard Research Center , Clamart ,","place":["France"]},{"name":"Ho\u0302pital Paul Brousse , Villejuif ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Franc\u0327oise","family":"Bourgeade","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 131 and Unite\u0301 542, and Claude Bernard Research Center , Clamart ,","place":["France"]},{"name":"Ho\u0302pital Paul Brousse , Villejuif ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aime\u0301","family":"Vazquez","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Unite\u0301 131 and Unite\u0301 542, and Claude Bernard Research Center , Clamart ,","place":["France"]},{"name":"Ho\u0302pital Paul Brousse , Villejuif ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,7,15]]},"reference":[{"key":"2026020209094866600_R1","first-page":"115","volume-title":"FEBS Lett.","year":"1996"},{"key":"2026020209094866600_R2","first-page":"1","volume-title":"Biochem. 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