{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T02:47:27Z","timestamp":1770086847290,"version":"3.49.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Granulomatous experimental autoimmune thyroiditis (G-EAT) is induced by transfer of mouse thyroglobulin (MTg)-sensitized spleen cells activated in vitro with MTg and anti-IL-2R or MTg and IL-12. Previous work suggested that IL-12 was required in vitro for development of G-EAT. To determine whether IL-12 was also required during the induction and\/or effector phases, DBA\/1 mice with a disrupted IL-12-P40 gene (IL-12\u2212\/\u2212) were used for EAT induction. Cells from MTg-sensitized IL12\u2212\/\u2212 donors activated in vitro by MTg or MTg and anti-IL2R induced severe EAT in recipient mice. Compared with effector cells from IL-12+\/+ donors, effector cells from IL-12\u2212\/\u2212 donors induced thyroid lesions dominated by lymphocytes with minimal granulomatous changes. Thyroids of recipients of IL-12\u2212\/\u2212 cells expressed less IFN-\u03b3 mRNA and more TGF-\u03b2, IL-4, and IL-10 compared with recipients of IL-12+\/+ cells. When IL-12 was added during in vitro activation, cells from both IL-12\u2212\/\u2212 and IL-12+\/+ donors induced severe G-EAT, and expression of all cytokines except IL-12 was comparable in thyroids of both IL-12+\/+ and IL-12\u2212\/\u2212 recipients. Transfer of cells from IL-12+\/+ or IL-12\u2212\/\u2212 donors into IL-12+\/+ or IL-12\u2212\/\u2212 recipients indicated that IL-12 expressed in thyroids was derived from recipients. Thus, endogenous IL-12 is not absolutely essential for the sensitization and activation of EAT effector cells to induce severe EAT, although it is required in vitro to promote activation of cells to induce severe granulomatous histopathology.<\/jats:p>","DOI":"10.4049\/jimmunol.167.3.1720","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T23:31:49Z","timestamp":1398123109000},"page":"1720-1727","source":"Crossref","is-referenced-by-count":11,"title":["Induction of Experimental Autoimmune Thyroiditis in IL-12\u2212\/\u2212 Mice"],"prefix":"10.1093","volume":"167","author":[{"given":"Kemin","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University of Missouri , Columbia, MO 65212"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongzhong","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Missouri , Columbia, MO 65212"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon C","family":"Sharp","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Missouri , Columbia, MO 65212"},{"name":"Department of Pathology, School of Medicine, University of Missouri , Columbia, MO 65212"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Braley-Mullen","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Missouri , Columbia, MO 65212"},{"name":"Department of Molecular Microbiology and Immunology, University of Missouri , Columbia, MO 65212"},{"name":"Department of Veteran\u2019s Affairs Research Service , Columbia, MO 65212"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,8,1]]},"reference":[{"key":"2026020209132065200_R1","first-page":"263","volume-title":"Adv. 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