{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T02:24:23Z","timestamp":1770085463364,"version":"3.49.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We examined TCR usage to a protective fragment of heat shock protein 60 from the fungus, Histoplasma capsulatum. Nearly 90% of T cell clones from C57BL\/6 mice vaccinated with this protein were V\u03b26+; the remainder were V\u03b214+. Amino acid motifs of the CDR3 region from V\u03b26+ cells were predominantly IxGGG, IGG, or SxxGG, whereas it was uniformly SFSGG for V\u03b214+ clones. Short term T cell lines from V\u03b26+-depleted mice failed to recognize Ag, and no T cell clones could be generated. To determine whether V\u03b26+ cells were functionally important, we eliminated them during vaccination. Depletion of V\u03b26+ cells abrogated protection in vivo and upon adoptive transfer of cells into TCR \u03b1\u03b2\u2212\/\u2212 mice. Transfer of a V\u03b26+, but not a V\u03b214+, clone into TCR \u03b1\u03b2\u2212\/\u2212 mice prolonged survival. Cytokine generation by Ag-stimulated splenocytes from immunized mice depleted of V\u03b26+ cells was similar to that of controls. The efficacy of the V\u03b26+ clone was associated with elevated production of IFN-\u03b3, TNF-\u03b1, and GM-CSF compared with that of the V\u03b214+ clone. More V\u03b26+ cells were present in lungs and spleens of TCR \u03b1\u03b2\u2212\/\u2212 on day 3 postinfection compared with V\u03b214+ cells. Thus, a single V\u03b2 family was essential for vaccine-induced immunity. Moreover, the mechanism by which V\u03b26+ contributed to protective immunity differed between unfractionated splenocytes and T cell clones.<\/jats:p>","DOI":"10.4049\/jimmunol.167.4.2219","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T19:18:03Z","timestamp":1398194283000},"page":"2219-2226","source":"Crossref","is-referenced-by-count":10,"title":["V\u03b26+ T Cells Are Obligatory for Vaccine-Induced Immunity to\n                    <i>Histoplasma capsulatum<\/i>"],"prefix":"10.1093","volume":"167","author":[{"suffix":"Jr.","given":"George S","family":"Deepe","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, University of Cincinnati College of Medicine, and Veterans Affairs Hospital , Cincinnati, OH 45267"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reta","family":"Gibbons","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Cincinnati College of Medicine, and Veterans Affairs Hospital , Cincinnati, OH 45267"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,8,15]]},"reference":[{"key":"2026020209105263400_R1","first-page":"2718","volume-title":"Principles and Practice of Infectious Diseases","year":"2000"},{"key":"2026020209105263400_R2","first-page":"2587","volume-title":"Infect. 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