{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T10:31:09Z","timestamp":1778409069894,"version":"3.51.4"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The progressive disease following Leishmania amazonensis infection in mice requires functional CD4+ T cells, which are primed to a disease-promoting phenotype during the infection. To understand how these pathogenic T cells are generated and the role of dendritic cells (DCs) in this process, we use DCs of susceptible BALB\/c and resistant C3H\/HeJ mice to examine parasite-DC interactions in vitro as well as the effector phenotype of T cells primed by parasite-exposed DCs in vivo. Our results demonstrate that amastigotes and metacyclics efficiently enter and activate DCs of both genetic backgrounds. Infection with amastigotes fails to induce CD40-depedent IL-12 production, but rather potentiates IL-4 production in BALB\/c DCs. Upon transfer into syngeneic recipients, amastigote-exposed BALB\/c DCs prime parasite-specific Th cells to produce significantly higher levels of IL-4 and IL-10 than their C3H\/HeJ counterparts. Transfer studies with IL-4\u2212\/\u2212 DCs indicate that this enhanced Th2 priming seen in BALB\/c mice is partially due to the IL-4 production by amastigote-carrying DCs. These results suggest that L. amazonensis amastigotes may condition DCs of a susceptible host to a state that favors activation of pathogenic CD4+ T cells, and thereby provide a new perspective on the pathogenesis of cutaneous leishmaniasis and protozoan parasite-host interactions in general.<\/jats:p>","DOI":"10.4049\/jimmunol.167.8.4534","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T19:47:50Z","timestamp":1398023270000},"page":"4534-4542","source":"Crossref","is-referenced-by-count":80,"title":["<i>Leishmania amazonensis<\/i>\n                    -Dendritic Cell Interactions In Vitro and the Priming of Parasite-Specific CD4+ T Cells In Vivo"],"prefix":"10.1093","volume":"167","author":[{"given":"Hai","family":"Qi","sequence":"first","affiliation":[{"name":"Pathology, University of Texas Medical Branch , Galveston, TX 77555"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vsevolod","family":"Popov","sequence":"additional","affiliation":[{"name":"Pathology, University of Texas Medical Branch , Galveston, TX 77555"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynn","family":"Soong","sequence":"additional","affiliation":[{"name":"Pathology, University of Texas Medical Branch , Galveston, TX 77555"},{"name":"Microbiology and Immunology, University of Texas Medical Branch , Galveston, TX 77555"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,10,15]]},"reference":[{"key":"2026020208123367000_R1","first-page":"1417","volume-title":"Science","year":"1984"},{"key":"2026020208123367000_R2","first-page":"175","volume-title":"Adv. Parasitol.","year":"1992"},{"key":"2026020208123367000_R3","first-page":"1","volume-title":"Molecular and Immune Mechanisms in the Pathogenesis of Cutaneous Leishmaniasis. R.G. Landes Company, Austin, TX, p.","year":"1996"},{"key":"2026020208123367000_R4","first-page":"151","volume-title":"Annu. Rev. Immunol.","year":"1995"},{"key":"2026020208123367000_R5","first-page":"243","volume-title":"Immunobiology","year":"1996"},{"key":"2026020208123367000_R6","first-page":"1302","volume-title":"J. Infect. Dis.","year":"1995"},{"key":"2026020208123367000_R7","first-page":"395","volume-title":"J. Immunogenet.","year":"1983"},{"key":"2026020208123367000_R8","first-page":"1653","volume-title":"J. Immunol.","year":"1991"},{"key":"2026020208123367000_R9","first-page":"5374","volume-title":"J. Immunol.","year":"1997"},{"key":"2026020208123367000_R10","first-page":"2952","volume-title":"Infect. Immun.","year":"1993"},{"key":"2026020208123367000_R11","first-page":"515","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020208123367000_R12","first-page":"1389","volume-title":"Eur. J. Immunol.","year":"1998"},{"key":"2026020208123367000_R13","first-page":"2467","volume-title":"Eur. J. Immunol.","year":"1998"},{"key":"2026020208123367000_R14","first-page":"438","volume-title":"Curr. Opin. Microbiol.","year":"1999"},{"key":"2026020208123367000_R15","first-page":"1857","volume-title":"J. Immunol.","year":"1993"},{"key":"2026020208123367000_R16","first-page":"2050","volume-title":"J. Immunol.","year":"1993"},{"key":"2026020208123367000_R17","first-page":"3163","volume-title":"Eur. J. Immunol.","year":"1996"},{"key":"2026020208123367000_R18","first-page":"271","volume-title":"Annu. Rev. Immunol.","year":"1991"},{"key":"2026020208123367000_R19","first-page":"245","volume-title":"Nature","year":"1998"},{"key":"2026020208123367000_R20","first-page":"389","volume-title":"J. Exp. Med.","year":"1995"},{"key":"2026020208123367000_R21","first-page":"479","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2026020208123367000_R22","first-page":"25","volume-title":"Immunol. Rev.","year":"1997"},{"key":"2026020208123367000_R23","first-page":"561","volume-title":"Immunol. Today","year":"1999"},{"key":"2026020208123367000_R24","first-page":"41","volume-title":"Trends Immunol.","year":"2001"},{"key":"2026020208123367000_R25","first-page":"383","volume-title":"Science","year":"1992"},{"key":"2026020208123367000_R26","first-page":"801","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2026020208123367000_R27","first-page":"73","volume-title":"Nature","year":"1999"},{"key":"2026020208123367000_R28","first-page":"4033","volume-title":"Infect. Immun.","year":"1999"},{"key":"2026020208123367000_R29","first-page":"762","volume-title":"Eur. J. Immunol.","year":"1999"},{"key":"2026020208123367000_R30","first-page":"1693","volume-title":"J. Exp. Med.","year":"1992"},{"key":"2026020208123367000_R31","first-page":"77","volume-title":"J. Immunol. Methods","year":"1999"},{"key":"2026020208123367000_R32","first-page":"319","volume-title":"Immunity","year":"1996"},{"key":"2026020208123367000_R33","first-page":"631","volume-title":"J. Exp. Med.","year":"1990"},{"key":"2026020208123367000_R34","first-page":"1547","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020208123367000_R35","first-page":"5858","volume-title":"J. Immunol.","year":"2000"},{"key":"2026020208123367000_R36","first-page":"161","volume-title":"Exp. Parasitol.","year":"1998"},{"key":"2026020208123367000_R37","first-page":"1666","volume-title":"J. Immunol.","year":"1998"},{"key":"2026020208123367000_R38","first-page":"310","volume-title":"J. Leukocyte Biol.","year":"1995"},{"key":"2026020208123367000_R39","first-page":"747","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020208123367000_R40","first-page":"741","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026020208123367000_R41","first-page":"3559","volume-title":"Infect. Immun.","year":"1995"},{"key":"2026020208123367000_R42","first-page":"693","volume-title":"Eur. J. Immunol.","year":"1995"},{"key":"2026020208123367000_R43","first-page":"969","volume-title":"J. Immunol.","year":"2000"},{"key":"2026020208123367000_R44","first-page":"5176","volume-title":"Infect. Immun.","year":"2000"},{"key":"2026020208123367000_R45","first-page":"297","volume-title":"Annu. Rev. Immunol.","year":"1997"},{"key":"2026020208123367000_R46","first-page":"1213","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026020208123367000_R47","first-page":"233","volume-title":"J. Exp. Med.","year":"2001"},{"key":"2026020208123367000_R48","first-page":"876","volume-title":"Eur. J. Immunol.","year":"2001"},{"key":"2026020208123367000_R49","first-page":"635","volume-title":"Annu. Rev. Immunol.","year":"1994"},{"key":"2026020208123367000_R50","first-page":"1797","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2026020208123367000_R51","first-page":"1553","volume-title":"Eur. J. Immunol.","year":"1996"},{"key":"2026020208123367000_R52","first-page":"364","volume-title":"J. Immunol.","year":"2000"},{"key":"2026020208123367000_R53","first-page":"229","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2026020208123367000_R54","first-page":"1661","volume-title":"J. Exp. Med.","year":"2000"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/167\/8\/4534\/62540815\/4534.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/167\/8\/4534\/62540815\/4534.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T13:20:57Z","timestamp":1770038457000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/167\/8\/4534\/8017403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10]]},"references-count":54,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2001,10,15]]},"published-print":{"date-parts":[[2001,10,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.167.8.4534","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2001,10]]},"published":{"date-parts":[[2001,10]]}}}