{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T17:05:45Z","timestamp":1778864745755,"version":"3.51.4"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2002,5,1]],"date-time":"2002-05-01T00:00:00Z","timestamp":1020211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Phenobarbital has a high potential to elicit adverse reactions including severe skin eruptions and systemic involvements among the worldwide-prescribed drugs. Although phenobarbital hypersensitivity is thought to be mediated by T cells specific to the drug, its precise mechanism remains not fully elucidated. To characterize T cells reactive with phenobarbital, we generated drug-specific T cell clones and lines from PBMCs of patients with phenobarbital hypersensitivity showing various degrees of cutaneous and extracutaneous involvements. Although the TCR V\u03b2 repertoire and phenotype in the T cell clones\/T cell lines were heterogeneous among the patients, V\u03b213.1+ and V\u03b25.1+ clones or lines were raised from the individuals examined who possessed different HLA haplotypes. Histopathological examination suggested that V\u03b25.1+CD8+ T cells and V\u03b213.1+ T cells played a role in cutaneous and extracutaneous involvements, respectively. A V\u03b213.1+CD4+ clone was found to proliferate in response to the Ag with processing-impaired, fixed APCs. Most of the clones and lines belonged to the Th2 phenotype, producing IL-4 and IL-5 but not IFN-\u03b3 upon phenobarbital stimulation. Clones\/lines with Th1 or Th0 phenotypes also constituted minor populations. These observations clearly indicate the heterogeneity and a marked individual deviation of reactive T cell subsets among the patients in terms of CD4\/8 phenotype, V\u03b2 repertoire, Ag recognition pattern, and cytokine production; and thus provide evidence whereby each pathogenic T cell subset contributes to special elements of clinical presentation.<\/jats:p>","DOI":"10.4049\/jimmunol.168.10.5359","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T18:26:04Z","timestamp":1398018364000},"page":"5359-5368","source":"Crossref","is-referenced-by-count":52,"title":["Characterization of Drug-Specific T Cells in Phenobarbital- Induced Eruption"],"prefix":"10.1093","volume":"168","author":[{"given":"Hideo","family":"Hashizume","sequence":"first","affiliation":[{"name":"Department of Dermatology, Hamamatsu University School of Medicine , Hamamatsu ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Takigawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Hamamatsu University School of Medicine , Hamamatsu ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiki","family":"Tokura","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Hamamatsu University School of Medicine , Hamamatsu ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,5,15]]},"reference":[{"key":"2026020208204180400_R1","first-page":"2190","volume-title":"Lancet","year":"1999"},{"key":"2026020208204180400_R2","first-page":"157","volume-title":"J. 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