{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:27:07Z","timestamp":1790202427324,"version":"4.1.0"},"reference-count":72,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Myelin oligodendrocyte glycoprotein (MOG) is an encephalitogenic myelin protein and a likely autoantigen in human multiple sclerosis (MS). In this work, we describe the fine specificity and cytokine profile of T cell clones (TCC) directed against MOG in three nuclear families, comprised of four individuals affected with MS and their HLA-identical siblings. TCC were generated from PBMC by limiting dilution against a mixture of eleven 20-mer overlapping peptides corresponding to the encephalitogenic extracellular domain of human MOG (aa 1\u2013120). The frequency of MOG peptide-reactive T cells was surprisingly high (range, 1:400 to 1:3,000) and, unexpectedly, cloning efficiencies were highest at low seeding densities of 102 or 103 PBMC per well. A total of 235 MOG peptide-reactive TCC were produced, all of which were CD4+CD8\u2212TCR\u03b1\u03b2+TCR\u03b3\u03b4\u2212. All 11 MOG peptides were recognized by the TCC, and different epitopes of MOG appeared to be immunodominant in the HLA-identical siblings. The patterns of cytokine secretion by TCC from single individuals were generally similar. The healthy individuals exhibited Th2-, Th0-, and T regulatory cell 1-like cytokine profiles, whereas TCC from one sibling with MS had a striking Th1-like phenotype, producing high levels of IFN-\u03b3 and TNF-\u03b1, and low IL-4 levels. Thus, MOG-reactive T cells appear to constitute an important part of the natural T cell repertoire, a finding that could contribute to the development of autoimmunity to this protein.<\/jats:p>","DOI":"10.4049\/jimmunol.168.11.5920","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T22:43:29Z","timestamp":1398120209000},"page":"5920-5927","source":"Crossref","is-referenced-by-count":35,"title":["The Human T Cell Response to Myelin Oligodendrocyte Glycoprotein: A Multiple Sclerosis Family-Based Study"],"prefix":"10.1093","volume":"168","author":[{"given":"Niklas K U","family":"Koehler","sequence":"first","affiliation":[{"name":"Department of Neurology, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude P","family":"Genain","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Giesser","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Arizona , Tucson, AZ 85724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L","family":"Hauser","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,6,1]]},"reference":[{"key":"2026020208201769600_R1","first-page":"299","volume-title":"Cell","year":"1996"},{"key":"2026020208201769600_R2","first-page":"101","volume-title":"Annu. 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