{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:18:30Z","timestamp":1791134310032,"version":"4.1.0"},"reference-count":67,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>B, \u03b1\u03b2 T, and NK lymphocytes establish immunological synapses (IS) with their targets to enable recognition. Transfer of target cell-derived Ags together with proximal molecules onto the effector cell appears also to occur through synapses. Little is known about the molecular basis of this transfer, but it is assumed to result from Ag receptor internalization. Because human \u03b3\u03b4 T cells recognize soluble nonpeptidic phosphoantigens as well as tumor cells such as Daudi, it is unknown whether they establish IS with, and extract molecules from, target cells. Using flow cytometry and confocal microscopy, we show in this work that Ag-stimulated human V\u03b39\/V\u03b42 T cells conjugate to, and perform molecular transfer from, various tumor cell targets. The molecular transfer appears to be linked to IS establishment, evolves in a dose-dependent manner in the presence of either soluble or cellular Ag, and requires \u03b3\u03b4 TCR ligation, Src family kinase signaling, and participation of the actin cytoskeleton. Although CD45 exclusion characterized the IS performed by \u03b3\u03b4 T cells, no obvious capping of the \u03b3\u03b4 TCR was detected. The synaptic transfer mediated by \u03b3\u03b4 T cells involved target molecules unrelated to the cognate Ag and occurred independently of MHC class I expression by target cells. From these observations, we conclude thatm despite the particular features of \u03b3\u03b4 T cell activation, both synapse formation and molecular transfer of determinants belonging to target cell characterize \u03b3\u03b4 T cell recognition of Ags.<\/jats:p>","DOI":"10.4049\/jimmunol.168.12.6336","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T22:38:18Z","timestamp":1398206298000},"page":"6336-6343","source":"Crossref","is-referenced-by-count":49,"title":["Synaptic Transfer by Human \u03b3\u03b4 T Cells Stimulated with Soluble or Cellular Antigens"],"prefix":"10.1093","volume":"168","author":[{"given":"Eric","family":"Espinosa","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Centre Hospitalier Universitaire Purpan , Toulouse ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Tabiasco","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Centre Hospitalier Universitaire Purpan , Toulouse ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Hudrisier","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Centre Hospitalier Universitaire Purpan , Toulouse ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Fournie\u0301","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Centre Hospitalier Universitaire Purpan , Toulouse ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,6,15]]},"reference":[{"key":"2026020209034187700_R1","first-page":"82","volume-title":"Nature","year":"1998"},{"key":"2026020209034187700_R2","first-page":"649","volume-title":"Science","year":"1999"},{"key":"2026020209034187700_R3","first-page":"221","volume-title":"Science","year":"1999"},{"key":"2026020209034187700_R4","first-page":"375","volume-title":"Annu. 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