{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:36:52Z","timestamp":1791297412727,"version":"4.1.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a newly assigned member of the Ig-immunoreceptor tyrosine-based inhibitory motif superfamily, and its functional role is suggested to be an inhibitory receptor that modulates immunoreceptor tyrosine-based activation motif-dependent signaling cascades. In this study, we hypothesized that PECAM-1 plays an essential in vivo role as a counterregulator of immediate hypersensitivity reactions. We found that PECAM-1 was highly expressed on the surface of immature bone marrow mast cells and at a lower density on mature peritoneal mast cells. Examination of skin biopsies from PECAM-1+\/+ and PECAM-1\u2212\/\u2212 mice revealed that absence of PECAM-1 did not affect mast cell development or the capacity of mast cells to populate tissues. To examine whether the absence of PECAM-1 would influence immediate hypersensitivity reactions, PECAM-1+\/+ and PECAM-1\u2212\/\u2212 mice were presensitized with anti-DNP mouse IgE and then challenged 20 h later with DNP-BSA or PBS. PECAM-1\u2212\/\u2212 mice exhibited elevated serum histamine concentrations after Ag stimulation compared with PECAM-1+\/+ mice, indicating an increased severity of systemic IgE-mediated anaphylaxis. PECAM-1\u2212\/\u2212 mice have increased sensitivity to local cutaneous IgE-dependent anaphylaxis compared with PECAM-1+\/+ mice, as assessed by greater tissue swelling of their ears and mast cell degranulation in situ. PECAM-1\u2212\/\u2212 bone marrow mast cells showed enhanced dense granule serotonin release after Fc\u03b5RI cross-linking in vitro. These results suggest that PECAM-1 acts as a counterregulator in allergic disease susceptibility and severity and negatively modulates mast cell activation.<\/jats:p>","DOI":"10.4049\/jimmunol.168.12.6455","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T22:38:18Z","timestamp":1398206298000},"page":"6455-6462","source":"Crossref","is-referenced-by-count":50,"title":["Absence of Platelet Endothelial Cell Adhesion Molecule-1 (CD31) Leads to Increased Severity of Local and Systemic IgE-Mediated Anaphylaxis and Modulation of Mast Cell Activation"],"prefix":"10.1093","volume":"168","author":[{"given":"Mae-Xhum","family":"Wong","sequence":"first","affiliation":[{"name":"Division of Hematology, Hanson Institute, Institute of Medical and Veterinary Science , Adelaide, South Australia ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Roberts","sequence":"additional","affiliation":[{"name":"Division of Hematology, Hanson Institute, Institute of Medical and Veterinary Science , Adelaide, South Australia ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A","family":"Bartley","sequence":"additional","affiliation":[{"name":"Division of Hematology, Hanson Institute, Institute of Medical and Veterinary Science , Adelaide, South Australia ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise E","family":"Jackson","sequence":"additional","affiliation":[{"name":"Division of Hematology, Hanson Institute, Institute of Medical and Veterinary Science , Adelaide, South Australia ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,6,15]]},"reference":[{"key":"2026020208203989000_R1","first-page":"B31","volume-title":"Nature","year":"1999"},{"key":"2026020208203989000_R2","first-page":"103","volume-title":"Allergy","year":"1999"},{"key":"2026020208203989000_R3","first-page":"3071","volume-title":"J. 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