{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:14:05Z","timestamp":1791278045212,"version":"4.1.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Recent data show that proinflammatory stimuli may modify significantly ion transport in the airway epithelium and therefore the properties of the airway surface fluid. We have studied the effect of IL-4, a cytokine involved in the pathogenesis of asthma, on transepithelial ion transport in the human bronchial epithelium in vitro. Incubation of polarized bronchial epithelial cells with IL-4 for 6\u201348 h causes a marked inhibition of the amiloride-sensitive Na+ channel as measured in short circuit current experiments. On the other hand, IL-4 evokes a 2-fold increase in the current activated by a cAMP analog, which reflects the activity of the cystic fibrosis transmembrane conductance regulator (CFTR). Similarly, IL-4 enhances the response to apical UTP, an agonist that activates Ca2+-dependent Cl\u2212 channels. These effects are mimicked by IL-13 and blocked by an antagonist of IL-4R\u03b1. RT-PCR experiments show that IL-4 elicits a 7-fold decrease in the level of the \u03b3 amiloride-sensitive Na+ channel mRNA, one of the subunits of the amiloride-sensitive Na+ channel, and an increase in CFTR mRNA. Our data suggest that IL-4 may favor the hydration of the airway surface by decreasing Na+ absorption and increasing Cl\u2212 secretion. This could be required to fluidify the mucus, which is hypersecreted during inflammatory conditions. On the other hand, the modifications of ion transport could also affect the ion composition of airway surface fluid.<\/jats:p>","DOI":"10.4049\/jimmunol.168.2.839","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T18:18:01Z","timestamp":1398017881000},"page":"839-845","source":"Crossref","is-referenced-by-count":119,"title":["IL-4 Is a Potent Modulator of Ion Transport in the Human Bronchial Epithelium In Vitro"],"prefix":"10.1093","volume":"168","author":[{"given":"Luis J V","family":"Galietta","sequence":"first","affiliation":[{"name":"Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini , Genova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Pagesy","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et la Recherche Me\u0301dicale, Unite\u0301 468, Service de Biochimie et Ge\u0301ne\u0301tique, Ho\u0302pital Henri Mondor , Cre\u0301teil ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Folli","sequence":"additional","affiliation":[{"name":"Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini , Genova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Caci","sequence":"additional","affiliation":[{"name":"Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini , Genova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Romio","sequence":"additional","affiliation":[{"name":"Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini , Genova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Costes","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et la Recherche Me\u0301dicale, Unite\u0301 468, Service de Biochimie et Ge\u0301ne\u0301tique, Ho\u0302pital Henri Mondor , Cre\u0301teil ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Nicolis","sequence":"additional","affiliation":[{"name":"Laboratorio di Patologia Molecolare, Centro Regionale Fibrosi Cistica , Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Cabrini","sequence":"additional","affiliation":[{"name":"Laboratorio di Patologia Molecolare, Centro Regionale Fibrosi Cistica , Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Goossens","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et la Recherche Me\u0301dicale, Unite\u0301 468, Service de Biochimie et Ge\u0301ne\u0301tique, Ho\u0302pital Henri Mondor , Cre\u0301teil ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Ravazzolo","sequence":"additional","affiliation":[{"name":"Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini , Genova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Zegarra-Moran","sequence":"additional","affiliation":[{"name":"Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini , Genova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,1,15]]},"reference":[{"key":"2026020208153310500_R1","first-page":"271","volume-title":"Am. 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