{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:35:44Z","timestamp":1770910544567,"version":"3.50.1"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We have previously reported the isolation of the human matrix metalloproteinase (MMP)-19 (also referred to as RASI) from a synovium of a patient suffering from rheumatoid arthritis and its expression at the cell surface of activated PBMC. In this study, we have analyzed the regulation and cell surface expression of human MMP-19 in several human cell lines and blood-derived cells. Among the cell lines analyzed, MMP-19 is largely expressed by lung fibroblasts as well as by myeloid cell lines THP-1 and HL-60. After fractionating PBMC into CD14\u2212 and CD14+ populations we found that only the latter one expresses MMP-19. Although the myeloid cell lines as well as CD14+ cells express MMP-19 without stimulation, its production can be up-regulated by phorbol esters (PMA) or by adhesion. The adhesion-dependent expression was down-regulated or even abrogated by blockade of adhesion or interfering with adhesion-controlling signaling using \u03b1-tocopherol. We have shown that MMP-19 associates with the cell surface of myeloid cells. This cell surface association was not affected by phospholipase C. However, acidic treatment of the THP-1-derived cell membranes abolished the immunoprecipitation of MMP-19 thereof. Moreover, a high salt treatment of THP-1 cells diminished the MMP-19 detection on the cell surface. This implicates a noncovalent attachment of MMP-19 to the cell surface. Because a truncated form of the MMP-19, in which the hemopexin-like domain was deleted (\u0394hpMMP-19), does not associate with the surface, the hemopexin-like domain appears to be critical for the cell surface attachment of human MMP-19.<\/jats:p>","DOI":"10.4049\/jimmunol.168.3.1244","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:08:45Z","timestamp":1398024525000},"page":"1244-1251","source":"Crossref","is-referenced-by-count":42,"title":["Matrix Metalloproteinase-19 Is Expressed in Myeloid Cells in an Adhesion-Dependent Manner and Associates with the Cell Surface"],"prefix":"10.1093","volume":"168","author":[{"given":"Simon","family":"Mauch","sequence":"first","affiliation":[{"name":"Department of Immunology, University of Konstanz , Konstanz ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Kolb","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Konstanz , Konstanz ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Kolb","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Konstanz , Konstanz ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thorsten","family":"Sadowski","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Konstanz , Konstanz ,","place":["Germany"]},{"name":"Institute of Biochemistry, Christian-Albrechts University of Kiel , Kiel ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radislav","family":"Sedlacek","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Konstanz , Konstanz ,","place":["Germany"]},{"name":"Institute of Biochemistry, Christian-Albrechts University of Kiel , Kiel ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,2,1]]},"reference":[{"key":"2026020208162075800_R1","first-page":"362","volume-title":"Matrix Metalloproteinases: Biology of Extracellular Matrix Series","year":"1998"},{"key":"2026020208162075800_R2","first-page":"197","volume-title":"Crit. 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