{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T02:36:23Z","timestamp":1770086183029,"version":"3.49.0"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Live recombinant vaccines expressing defined pathogen-derived Ags represent powerful candidates for future vaccination strategies. In this study, we report on the differential induction of protective cell-mediated immunity elicited by different recombinant Mycobacterium bovis Bacille Calmette-Gue\u0301rin (BCG) strains displaying p60 Ag of Listeria monocytogenes in secreted, cytosolic, or membrane-attached form for T cell recognition. Anti-listerial protection evoked by the membrane-linked p60 lipoprotein of rBCG Mp60 and that of the p60 derivative secreted by rBCG Sp60-40 were nearly equal, whereas cytosolic p60 displayed by rBCG Np60 failed to protect mice from listeriosis. In vivo depletion of CD4 or CD8 T cell subpopulations in rBCG Mp60-vaccinated mice before listerial challenge revealed interactions of both T cell subsets in anti-listerial protection. In rBCG Sp60-40-vaccinated animals, CD4 T cells predominantly contributed to anti-listerial control as shown by the failure of anti-CD8 mAb treatment to impair the outcome of listeriosis in rBCG Sp60-40-vaccinated mice after L. monocytogenes challenge. Hence, differential Ag display by rBCG influences cell-mediated immunity, which in turn may impact vaccine efficacy due to the different requirements of CD4 or CD8 T cells for pathogen elimination.<\/jats:p>","DOI":"10.4049\/jimmunol.168.4.1869","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:56:53Z","timestamp":1398031013000},"page":"1869-1876","source":"Crossref","is-referenced-by-count":36,"title":["Cell-Mediated Immunity Induced by Recombinant\n                    <i>Mycobacterium bovis<\/i>\n                    Bacille Calmette-Gue\u0301rin Strains Against an Intracellular Bacterial Pathogen: Importance of Antigen Secretion or Membrane-Targeted Antigen Display as Lipoprotein for Vaccine Efficacy"],"prefix":"10.1093","volume":"168","author":[{"given":"Leander","family":"Grode","sequence":"first","affiliation":[{"name":"Department of Immunology, Max-Planck-Institute for Infection Biology , Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mischo","family":"Kursar","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max-Planck-Institute for Infection Biology , Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Fensterle","sequence":"additional","affiliation":[{"name":"Institute for Medical Radiation and Cell Research , Wurzburg ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan H E","family":"Kaufmann","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max-Planck-Institute for Infection Biology , Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju\u0308rgen","family":"Hess","sequence":"additional","affiliation":[{"name":"Department of Molecular Therapy, November AG , Erlangen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,2,15]]},"reference":[{"key":"2026020209044633100_R1","first-page":"955","volume-title":"Nat. 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