{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:51:23Z","timestamp":1770889883848,"version":"3.50.1"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Streptococcus pneumoniae is an important cause of upper and lower respiratory tract infections, meningitis, peritonitis, bacterial arthritis, and sepsis. Here we have studied a novel immune evasion mechanism of serotype 3 pneumococci, which are particularly resistant to phagocytosis. On their surfaces the bacteria express the factor H-binding inhibitor of complement (Hic), a protein of the pneumococcal surface protein C family. Using radioligand binding, microtiter plate assays, surface plasmon resonance analysis, and recombinant constructs of factor H, we located the binding site of Hic to short consensus repeats (SCRs) 8\u201311 in the middle part of factor H. This represents a novel microbial interaction region on factor H. The only other ligand known so far for SCRs 8\u201311 of factor H is C-reactive protein (CRP), an acute phase protein that binds to the pneumococcal C-polysaccharide. The binding sites of Hic and CRP within the SCR8\u201311 region were different, however, because CRP did not inhibit the binding of Hic and required calcium for binding. Binding of factor H to Hic-expressing pneumococci promoted factor I-mediated cleavage of C3b and restricted phagocytosis of pneumococci. Thus, virulent pneumococci avoid complement attack and opsonophagocytosis by recruiting functionally active factor H with the Hic surface protein. Hic binds to a previously unrecognized microbial interaction site in the middle part of factor H.<\/jats:p>","DOI":"10.4049\/jimmunol.168.4.1886","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:56:53Z","timestamp":1398031013000},"page":"1886-1894","source":"Crossref","is-referenced-by-count":148,"title":["<i>Streptococcus pneumoniae<\/i>\n                    Evades Complement Attack and Opsonophagocytosis by Expressing the\n                    <i>pspC<\/i>\n                    Locus-Encoded Hic Protein That Binds to Short Consensus Repeats 8\u201311 of Factor H"],"prefix":"10.1093","volume":"168","author":[{"given":"Hanna","family":"Jarva","sequence":"first","affiliation":[{"name":"Haartman Institute, University of Helsinki and Helsinki University Central Hospital , Helsinki ,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Janulczyk","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, University of Lund , Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Hellwage","sequence":"additional","affiliation":[{"name":"Hans Knoell Institute for Natural Products Research , Jena ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F","family":"Zipfel","sequence":"additional","affiliation":[{"name":"Hans Knoell Institute for Natural Products Research , Jena ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Bjo\u0308rck","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, University of Lund , Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seppo","family":"Meri","sequence":"additional","affiliation":[{"name":"Haartman Institute, University of Helsinki and Helsinki University Central Hospital , Helsinki ,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,2,15]]},"reference":[{"key":"2026020209061877400_R1","first-page":"1385","volume-title":"Science","year":"1994"},{"key":"2026020209061877400_R2","first-page":"2759","volume-title":"J. 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