{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:36:00Z","timestamp":1761708960271,"version":"3.40.3"},"reference-count":70,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Intratracheal instillation of Sephadex particles is a convenient model for assessing the impact of potential anti-inflammatory compounds on lung eosinophilia thought to be a key feature in asthma pathophysiology. However, the underlying cellular and molecular mechanisms involved are poorly understood. We have studied the time course of Sephadex-induced lung eosinophilia, changes in pulmonary T cell numbers, and gene and protein expression as well as the immunological and pharmacological modulation of these inflammatory indices in the Sprague Dawley rat. Sephadex increased T cell numbers (including CD4+ T cells) and evoked a pulmonary eosinophilia that was associated with an increase in gene\/protein expression of the Th2-type cytokines IL-4, IL-5, and IL-13 and eotaxin in lung tissue. Sephadex instillation also induced airway hyperreactivity to acetylcholine and bradykinin. A neutralizing Ab (R73) against the \u03b1\u03b2-TCR caused 54% depletion of total (CD2+) pulmonary T cells accompanied by a significant inhibition of IL-4, IL-13 and eotaxin gene expression together with suppression (65% inhibition) of eosinophils in lung tissue 24 h after Sephadex treatment. Sephadex-induced eosinophilia and Th2 cytokine gene and\/or protein expression were sensitive to cyclosporin A and budesonide, compounds that inhibit T cell function, suggesting a pivotal role for T cells in orchestrating Sephadex-induced inflammation in this model.<\/jats:p>","DOI":"10.4049\/jimmunol.168.6.3004","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T00:31:13Z","timestamp":1398040273000},"page":"3004-3016","source":"Crossref","is-referenced-by-count":27,"title":["Critical Role for T Cells in Sephadex-Induced Airway Inflammation: Pharmacological and Immunological Characterization and Molecular Biomarker Identification"],"prefix":"10.1093","volume":"168","author":[{"given":"El-Bdaoui","family":"Haddad","sequence":"first","affiliation":[{"name":"Pharmacology Department , Dagenham ,","place":["United Kingdom"]},{"name":"Respiratory and RA Disease Group, Aventis Pharma , Bridgewater, NJ 08807"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen L","family":"Underwood","sequence":"additional","affiliation":[{"name":"Pharmacology Department , Dagenham ,","place":["United Kingdom"]},{"name":"Respiratory and RA Disease Group, Aventis Pharma , Bridgewater, NJ 08807"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominika","family":"Dabrowski","sequence":"additional","affiliation":[{"name":"Pharmacology Department , Dagenham ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A","family":"Birrell","sequence":"additional","affiliation":[{"name":"Respiratory Pharmacology, Cardiothoracic Surgery, Imperial College School of Medicine, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerryn","family":"McCluskie","sequence":"additional","affiliation":[{"name":"Respiratory Pharmacology, Cardiothoracic Surgery, Imperial College School of Medicine, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cliff H","family":"Battram","sequence":"additional","affiliation":[{"name":"Novartis , Horsham ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Pecoraro","sequence":"additional","affiliation":[{"name":"Pharmacology Department , Dagenham ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martyn L","family":"Foster","sequence":"additional","affiliation":[{"name":"AstraZeneca , Charnwood ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria G","family":"Belvisi","sequence":"additional","affiliation":[{"name":"Respiratory Pharmacology, Cardiothoracic Surgery, Imperial College School of Medicine, National Heart and Lung Institute , London ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,3,15]]},"reference":[{"first-page":"3","volume-title":"Br. J. Clin. Pharmacol.","year":"1996","key":"2025032712351825900_R1"},{"first-page":"825","volume-title":"Thorax","year":"1999","key":"2025032712351825900_R2"},{"first-page":"310","volume-title":"Thorax","year":"1997","key":"2025032712351825900_R3"},{"first-page":"255","volume-title":"Annu. Rev. Immunol.","year":"1999","key":"2025032712351825900_R4"},{"first-page":"642","volume-title":"Am. J. Respir. Cell Mol. Biol.","year":"1997","key":"2025032712351825900_R5"},{"first-page":"247","volume-title":"Immunology","year":"1996","key":"2025032712351825900_R6"},{"first-page":"439","volume-title":"Br. J. Pharmacol.","year":"1997","key":"2025032712351825900_R7"},{"first-page":"821","volume-title":"Br. J. Pharmacol.","year":"1990","key":"2025032712351825900_R8"},{"first-page":"266","volume-title":"Int. Arch. Allergy Immunol.","year":"1992","key":"2025032712351825900_R9"},{"first-page":"355","volume-title":"Agent Actions","year":"1985","key":"2025032712351825900_R10"},{"first-page":"185","volume-title":"Pulml. Pharmacol.","year":"1989","key":"2025032712351825900_R11"},{"first-page":"363","volume-title":"Int. Arch. Allergy Appl. Immunol.","year":"1986","key":"2025032712351825900_R12"},{"first-page":"537","volume-title":"Inflammation","year":"1993","key":"2025032712351825900_R13"},{"first-page":"321","volume-title":"Clin. Immunol. Immunopathol.","year":"1994","key":"2025032712351825900_R14"},{"first-page":"233","volume-title":"Exp. Toxicol. Pathol.","year":"1996","key":"2025032712351825900_R15"},{"first-page":"523","volume-title":"Inflammation","year":"1996","key":"2025032712351825900_R16"},{"first-page":"299","volume-title":"Inflamm. Res.","year":"1997","key":"2025032712351825900_R17"},{"first-page":"437","volume-title":"Inflammation","year":"1999","key":"2025032712351825900_R18"},{"first-page":"27","volume-title":"Airways Smooth Muscle: Modelling the Inflammatory Response In Vivo","year":"1996","key":"2025032712351825900_R19"},{"first-page":"156","volume-title":"Anal. Biochem.","year":"1987","key":"2025032712351825900_R20"},{"first-page":"1262","volume-title":"Br. J. Pharmacol.","year":"1999","key":"2025032712351825900_R21"},{"first-page":"135","volume-title":"Arch. Int. Pharmacodyn. Ther.","year":"1984","key":"2025032712351825900_R22"},{"first-page":"1975","volume-title":"J. Immunol.","year":"2001","key":"2025032712351825900_R23"},{"first-page":"165","volume-title":"Eur. J. Pharmacol.","year":"2001","key":"2025032712351825900_R24"},{"first-page":"602","volume-title":"Inflamm. Res.","year":"1999","key":"2025032712351825900_R25"},{"first-page":"743","volume-title":"Eur. Respir. J.","year":"2000","key":"2025032712351825900_R26"},{"first-page":"67","volume-title":"Int. Arch. Allergy Immunol.","year":"1998","key":"2025032712351825900_R27"},{"first-page":"587","volume-title":"J. Clin. Invest.","year":"1996","key":"2025032712351825900_R28"},{"first-page":"173","volume-title":"Immunol. Rev.","year":"2001","key":"2025032712351825900_R29"},{"first-page":"20","volume-title":"Mol. Med. Today","year":"2000","key":"2025032712351825900_R30"},{"first-page":"788","volume-title":"Biochem. Biophys. Res. Commun.","year":"1994","key":"2025032712351825900_R31"},{"first-page":"1","volume-title":"Immunity","year":"1996","key":"2025032712351825900_R32"},{"first-page":"1461","volume-title":"J. Exp. Med.","year":"1996","key":"2025032712351825900_R33"},{"first-page":"6273","volume-title":"Proc. NY Acad. Sci.","year":"1998","key":"2025032712351825900_R34"},{"first-page":"785","volume-title":"J. Exp. Med.","year":"1997","key":"2025032712351825900_R35"},{"first-page":"177","volume-title":"Int. J. Exp. Pathol.","year":"1999","key":"2025032712351825900_R36"},{"first-page":"2001","volume-title":"Am. J. Pathol.","year":"1999","key":"2025032712351825900_R37"},{"first-page":"2143","volume-title":"J. Exp. Med.","year":"1997","key":"2025032712351825900_R38"},{"first-page":"548","volume-title":"Am. Rev. Respir. Dis.","year":"1993","key":"2025032712351825900_R39"},{"first-page":"54","volume-title":"Am. J. Respir. Cell Mol. Biol.","year":"1995","key":"2025032712351825900_R40"},{"first-page":"109","volume-title":"J. Exp. Med.","year":"1996","key":"2025032712351825900_R41"},{"first-page":"195","volume-title":"J. Exp. Med.","year":"1996","key":"2025032712351825900_R42"},{"first-page":"601","volume-title":"J. Exp. Med.","year":"1997","key":"2025032712351825900_R43"},{"first-page":"2142","volume-title":"J. Immunol.","year":"2000","key":"2025032712351825900_R44"},{"volume-title":"Immunology","key":"2025032712351825900_R45","first-page":"41"},{"first-page":"72s","volume-title":"Eur. Respir. J.","year":"1996","key":"2025032712351825900_R46"},{"first-page":"3796","volume-title":"J. Immunol.","year":"1990","key":"2025032712351825900_R47"},{"first-page":"78","volume-title":"J. Immunol.","year":"1999","key":"2025032712351825900_R48"},{"first-page":"920","volume-title":"J. Immunol.","year":"1999","key":"2025032712351825900_R49"},{"first-page":"2261","volume-title":"Science","year":"1998","key":"2025032712351825900_R50"},{"first-page":"1565","volume-title":"J. Exp. Med.","year":"1999","key":"2025032712351825900_R51"},{"first-page":"423","volume-title":"Immunity","year":"1998","key":"2025032712351825900_R52"},{"first-page":"399","volume-title":"J. Exp. Med.","year":"1998","key":"2025032712351825900_R53"},{"first-page":"254","volume-title":"Am. J. Respir. Cell Mol. Biol.","year":"1995","key":"2025032712351825900_R54"},{"first-page":"245","volume-title":"Nature","year":"1993","key":"2025032712351825900_R55"},{"first-page":"7821","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1996","key":"2025032712351825900_R56"},{"first-page":"2258","volume-title":"Science","year":"1998","key":"2025032712351825900_R57"},{"first-page":"5792","volume-title":"J. Immunol.","year":"2001","key":"2025032712351825900_R58"},{"first-page":"779","volume-title":"J. Clin. Invest.","year":"1999","key":"2025032712351825900_R59"},{"first-page":"73","volume-title":"J. Exp. Med.","year":"1989","key":"2025032712351825900_R60"},{"first-page":"4187","volume-title":"J. Immunol.","year":"1991","key":"2025032712351825900_R61"},{"first-page":"2805","volume-title":"Eur. J. Immunol.","year":"1990","key":"2025032712351825900_R62"},{"first-page":"3768","volume-title":"J. Immunol.","year":"1992","key":"2025032712351825900_R63"},{"first-page":"161","volume-title":"Agents Actions","year":"1991","key":"2025032712351825900_R64"},{"first-page":"S179","volume-title":"Am. J. Respir. Crit. Care Med.","year":"2000","key":"2025032712351825900_R65"},{"first-page":"73","volume-title":"Eur. Respir. J.","year":"1992","key":"2025032712351825900_R66"},{"first-page":"669","volume-title":"Am. Rev. Respir. Dis.","year":"1992","key":"2025032712351825900_R67"},{"first-page":"401","volume-title":"Am. Rev. Respir. Dis.","year":"1993","key":"2025032712351825900_R68"},{"first-page":"33","volume-title":"J. Exp. Med.","year":"1995","key":"2025032712351825900_R69"},{"first-page":"511","volume-title":"J. Allergy Clin. Immunol.","year":"1997","key":"2025032712351825900_R70"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/168\/6\/3004\/62550068\/3004.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/168\/6\/3004\/62550068\/3004.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T15:09:52Z","timestamp":1743347392000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/168\/6\/3004\/8025197"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,3]]},"references-count":70,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2002,3,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.168.6.3004","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"type":"print","value":"0022-1767"},{"type":"electronic","value":"1550-6606"}],"subject":[],"published-other":{"date-parts":[[2002,3]]},"published":{"date-parts":[[2002,3]]}}}