{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:56:21Z","timestamp":1791064581356,"version":"4.1.0"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Anti-DNA Abs commonly found in patients with systemic lupus erythematosus are thought to play an important pathogenic role in lupus nephritis. Anti-DNA Abs may contribute to renal disease by cross-reactivity with renal Ags, the identity of which remain elusive. To identify a target Ag for pathogenic anti-DNA Abs, we performed Western blotting and immunoprecipitations of mesangial cell lysates from the lupus-prone MRL-lpr\/lpr mouse and a nonautoimmune BALB\/c mouse with the pathogenic anti-DNA Ab R4A. We found that R4A (but not a nonpathogenic Ab mutant of R4A) binds to and immunoprecipitates a 100-kDa protein expressed on the cell surface and in lysates of MRL-lpr\/lpr mesangial cells. DNase treatment of the lysate and of the R4A Ab did not effect binding, indicating that the binding of R4A to the 100-kDa protein was direct and not mediated by an antigenic bridge containing DNA. Binding was greatly diminished in BALB\/c lysates, suggesting that Ag expression or availability at the level of the target organ may be a factor in determining susceptibility to lupus nephritis. Following identification of this 100-kDa protein as nonmuscle \u03b1-actinin, binding of R4A to \u03b1-actinin was confirmed by Western blot, ELISA, inhibition studies, and immunofluorescence. High titers of anti-\u03b1-actinin Abs were present in sera and kidney eluates of lupus mice with active nephritis. These results indicate that the nephritogenicity of some anti-DNA Abs may be mediated via cross-reactivity with \u03b1-actinin. Furthermore, variations in target Ag display between individuals may underlie differential susceptibility to anti-DNA Ab-induced renal disease.<\/jats:p>","DOI":"10.4049\/jimmunol.168.6.3072","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:31:13Z","timestamp":1398025873000},"page":"3072-3078","source":"Crossref","is-referenced-by-count":172,"title":["\u03b1-Actinin Is a Cross-Reactive Renal Target for Pathogenic Anti-DNA Antibodies"],"prefix":"10.1093","volume":"168","author":[{"given":"Bisram","family":"Deocharan","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Department of Medicine, Albert Einstein College of Medicine, Bronx , NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Qing","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, Albert Einstein College of Medicine , Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Lichauco","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, Albert Einstein College of Medicine , Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaim","family":"Putterman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Department of Medicine, Albert Einstein College of Medicine, Bronx , NY 10461"},{"name":"Division of Rheumatology, Department of Medicine, Albert Einstein College of Medicine , Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,3,15]]},"reference":[{"key":"2026020209073111400_R1","first-page":"1359","volume-title":"N. 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