{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T03:50:36Z","timestamp":1770090636578,"version":"3.49.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>CD47 modulates a variety of cell functions such as adhesion, spreading, and migration. Using a fusion protein consisting of the extracellular region of Src homology 2 domain bearing protein tyrosine phosphatase substrate-1 (SHPS-1) and the Fc portion of human Ig (SHPS-1-Ig) we investigated the effects of SHPS-1 as a ligand for CD47 on B lymphocytes. Although SHPS-1-Ig binding to human B cell lines was solely mediated via CD47, their binding capacity for soluble and immobilized SHPS-1-Ig varied among cell lines irrespective of the similar expression levels of CD47, suggesting that distinctive affinity\/avidity states exist during B cell maturation. Nalm6 cell line and tonsilar B lymphocytes adhered to immobilized SHPS-1-Ig and showed polarization-like morphology. These effects of SHPS-1-Ig were blocked by anti-CD47 mAbs (B6H12 and SE5A5). Wortmannin, a phosphatidylinositol-3 kinase inhibitor, but not pertussis toxin significantly inhibited the polarization induced by the immobilized SHPS-1-Ig. Thus, SHPS-1 acts as an adhesive substrate via CD47 in human B lymphocyte. Immunohistochemical analyses indicated that SHPS-1 is expressed on high endothelial venule as well as macrophages in human tonsils. HUVECs also express SHPS-1 in the absence of any stimuli, and the adhesion of tonsilar B lymphocytes to nonactivated HUVECs was significantly inhibited by SE5A5, indicating that SHPS-1\/CD47 interaction is involved in the adhesion. Our findings suggest that SHPS-1\/CD47 interaction may contribute to the recruitment of B lymphocytes via endothelial cells under steady state conditions.<\/jats:p>","DOI":"10.4049\/jimmunol.168.7.3213","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:08:54Z","timestamp":1398024534000},"page":"3213-3220","source":"Crossref","is-referenced-by-count":27,"title":["Interaction Between Src Homology 2 Domain Bearing Protein Tyrosine Phosphatase Substrate-1 and CD47 Mediates the Adhesion of Human B Lymphocytes to Nonactivated Endothelial Cells"],"prefix":"10.1093","volume":"168","author":[{"given":"Hitoshi","family":"Yoshida","sequence":"first","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Tomiyama","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Oritani","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Murayama","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisashi","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-ichiro","family":"Miyagawa","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nakayuki","family":"Honma","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Laboratories, Kirin Brewery Company , Gunma ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Nishiura","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Matsuzawa","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Molecular Science, Graduate School of Medicine B5, Osaka University , Osaka ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,4,1]]},"reference":[{"key":"2026020209102420700_R1","first-page":"501","volume-title":"EMBO J.","year":"1999"},{"key":"2026020209102420700_R2","first-page":"55","volume-title":"Autoimmunity","year":"1997"},{"key":"2026020209102420700_R3","first-page":"301","volume-title":"Cell","year":"1994"},{"key":"2026020209102420700_R4","first-page":"60","volume-title":"Science","year":"1996"},{"key":"2026020209102420700_R5","first-page":"343","volume-title":"J. 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