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Bacterially produced CD19 \u00d7 CD16 BsDb specifically interacted with both CD19+ and CD16+ cells and exhibited significantly higher apparent affinity and slower dissociation from the tumor cells than from effector cells. It was able to induce specific lysis of tumor cells in the presence of isolated human NK cells or nonfractionated PBLs. The combination of the CD19 \u00d7 CD16 BsDb with a previously described CD19 \u00d7 CD3 BsDb and CD28 costimulation significantly increased the lytic potential of human PBLs. Treatment of SCID mice bearing an established Burkitt\u2019s lymphoma (5 mm in diameter) with human PBLs, CD19 \u00d7 CD16 BsDb, CD19 \u00d7 CD3 BsDb, and anti-CD28 mAb resulted in the complete elimination of tumors in 80% of animals. In contrast, mice receiving human PBLs in combination with either diabody alone showed only partial tumor regression. These data clearly demonstrate the synergistic effect of small recombinant bispecific molecules recruiting different populations of human effector cells to the same tumor target.<\/jats:p>","DOI":"10.4049\/jimmunol.169.1.137","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:33:01Z","timestamp":1398025981000},"page":"137-144","source":"Crossref","is-referenced-by-count":75,"title":["Synergistic Antitumor Effect of Bispecific CD19 \u00d7 CD3 and CD19 \u00d7 CD16 Diabodies in a Preclinical Model of Non-Hodgkin\u2019s Lymphoma"],"prefix":"10.1093","volume":"169","author":[{"given":"Sergey M","family":"Kipriyanov","sequence":"first","affiliation":[{"name":"Affimed Therapeutics , Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjo\u0308rn","family":"Cochlovius","sequence":"additional","affiliation":[{"name":"Recombinant Ab Research Group, German Cancer Research Center , Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger J","family":"Scha\u0308fer","sequence":"additional","affiliation":[{"name":"Affimed Therapeutics , Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Moldenhauer","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, German Cancer Research Center , Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Ba\u0308hre","sequence":"additional","affiliation":[{"name":"Recombinant Ab Research Group, German Cancer Research Center , Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Le Gall","sequence":"additional","affiliation":[{"name":"Affimed Therapeutics , Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Knackmuss","sequence":"additional","affiliation":[{"name":"Affimed Therapeutics , Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melvyn","family":"Little","sequence":"additional","affiliation":[{"name":"Affimed Therapeutics , Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,7,1]]},"reference":[{"key":"2026020208192443600_R1","first-page":"3909","volume-title":"Blood","year":"1997"},{"key":"2026020208192443600_R2","first-page":"824","volume-title":"J. 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