{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:30:20Z","timestamp":1790267420163,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To investigate the role of \u03b3\u03b4 T cells in human autoimmune disease we expressed and characterized a \u03b3\u03b4 TCR from an autoimmune tissue lesion. The TCR was first identified in a rare form of polymyositis characterized by a monoclonal infiltrate of \u03b3\u03b4 T cells which invaded and destroyed skeletal muscle fibers. The V\u03b31.3-J\u03b31-C\u03b31\/V\u03b42-J\u03b43 TCR cDNA of the original muscle invasive \u03b3\u03b4 T cell clone was reconstructed from unrelated cDNA and transfected into the mouse hybridoma BW58\u03b1\u2212\u03b2\u2212. Appropriate anti-human \u03b3\u03b4 TCR Abs stimulated the TCR transfectants to produce IL-2, thus demonstrating that the human \u03b3\u03b4 TCR functionally interacted with murine signaling components. The transfected V\u03b31.3\/V\u03b42 TCR recognized a cytosolic protein expressed in cultured human myoblasts and TE671 rhabdomyosarcoma cells. The Ag was recognized in the absence of presenting cells. Using a panel of control \u03b3\u03b4 TCR transfectants with defined exchanges in different positions of both TCR chains, we showed that the \u03b3\u03b4 TCR recognized its Ag in a TCR complementarity-determining region 3-dependent way. To our knowledge, this is the first example of a molecularly defined \u03b3\u03b4 TCR directly derived from an autoimmune tissue lesion. The strategy used in this study may be applicable to other autoimmune diseases.<\/jats:p>","DOI":"10.4049\/jimmunol.169.1.515","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:33:01Z","timestamp":1398025981000},"page":"515-521","source":"Crossref","is-referenced-by-count":36,"title":["An Autoreactive \u03b3\u03b4 TCR Derived from a Polymyositis Lesion"],"prefix":"10.1093","volume":"169","author":[{"given":"Heinz","family":"Wiendl","sequence":"first","affiliation":[{"name":"Max Planck Institute of Neurobiology , Martinsried ,","place":["Germany"]},{"name":"Department of Neurology, University of Tu\u0308bingen , Tu\u0308bingen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Malotka","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Neurobiology , Martinsried ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Holzwarth","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Neurobiology , Martinsried ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Ulrich","family":"Weltzien","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Immunobiology , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartmut","family":"Wekerle","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Neurobiology , Martinsried ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Hohlfeld","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Neurobiology , Martinsried ,","place":["Germany"]},{"name":"Institute for Clinical Neuroimmunology, Klinikum Gro\u03b2hadern, Ludwig Maximilians University , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Dornmair","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Neurobiology , Martinsried ,","place":["Germany"]},{"name":"Institute for Clinical Neuroimmunology, Klinikum Gro\u03b2hadern, Ludwig Maximilians University , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,7,1]]},"reference":[{"key":"2026020208195365100_R1","first-page":"68","volume-title":"Nature","year":"1993"},{"key":"2026020208195365100_R2","first-page":"393","volume-title":"J. 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