{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:15:39Z","timestamp":1791231339812,"version":"4.1.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tumor-specific CD8 T cell responses to MCA102 fibrosarcoma cells expressing the cytotoxic T cell epitope gp33 from lymphocytic choriomeningitis virus were studied. MCA102gp33 tumors grew progressively in C57BL\/6 mice, despite induction of peripheral gp33-tetramer+ T cells that were capable of mediating antiviral protection, specific cell rejection, and concomitant tumor immunity. MCA102gp33 tumors were infiltrated with a high number (\u223c20%) of CD11b+CD11c\u2212 macrophage-phenotype cells that were able to cross-present the gp33 epitope to T cells. Tumor-infiltrating CD8 T cells exhibited a highly activated phenotype but lacked effector cell function. Strikingly, a significant portion of tumor-infiltrating lymphocytes expressed TCRs specific for gp33 but bound MHC tetramers only after cell purification and a 24-h resting period in vitro. The phenomenon of \u201ctetramer-negative T cells\u201d was not restricted to tumor-infiltrating lymphocytes from MCA102gp33 tumors, but was also observed when Ag-specific T cells derived from an environment with high Ag load were analyzed ex vivo. Thus, using a novel tumor model, allowing us to trace tumor-specific T cells at the single cell level in vivo, we demonstrate that the tumor microenvironment is able to alter the functional activity of T cells infiltrating the tumor mass.<\/jats:p>","DOI":"10.4049\/jimmunol.169.10.5522","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:04:08Z","timestamp":1398024248000},"page":"5522-5530","source":"Crossref","is-referenced-by-count":50,"title":["Lack of Effector Cell Function and Altered Tetramer Binding of Tumor-Infiltrating Lymphocytes"],"prefix":"10.1093","volume":"169","author":[{"given":"Ulrike","family":"Blohm","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Roth","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Brommer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilman","family":"Dumrese","sequence":"additional","affiliation":[{"name":"Institute of Experimental Immunology, University Hospital , Zurich ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felicia M","family":"Rosenthal","sequence":"additional","affiliation":[{"name":"Cell Genix Technologie Transfer GmbH , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg , Freiburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,11,15]]},"reference":[{"key":"2026020209204227500_R1","first-page":"1","volume-title":"Int. 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