{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:33:15Z","timestamp":1791099195352,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We have characterized dendritic cell (DC)-associated lectin-1 (DCAL-1), a novel, type II, transmembrane, C-type lectin-like protein. DCAL-1 has restricted expression in hemopoietic cells, in particular, DCs and B cells, but T cells and monocytes do not express it. The DCAL-1 locus is within a cluster of C-type lectin-like loci on human chromosome 12p12\u201313 just 3\u2032 to the CD69 locus. The consensus sequence of the DCAL-1 gene was confirmed by RACE-PCR; however, based on sequence alignment with genomic DNA and with various human expressed sequence tags, we predict that DCAL-1 has two splice variants. C-type lectins share a common sequence motif of 14 invariable and 18 highly conserved aa residues known as the carbohydrate recognition domain. DCAL-1, however, is missing three of the cysteine residues required to form the standard carbohydrate recognition domain. DCAL-1 mRNA and protein expression are increased upon the differentiation of monocytes to CD1a+ DCs. B cells also express high levels of DCAL-1 on their cell surface. Using a DCAL-1 fusion protein we identified a population of CD4+ CD45RA+ T cells that express DCAL-1 ligand. Coincubation with soluble DCAL-1 enhanced the proliferation of CD4+ T cells in response to CD3 ligation and significantly increased IL-4 secretion. In contrast, coincubation with soluble DC-specific ICAM-3-grabbing nonintegrin (CD209) fusion protein as a control had no effect on CD4+ T cell proliferation or IL-4 and IFN-\u03b3 secretion. Therefore, the function of DCAL-1 on DCs and B cells may act as a T cell costimulatory molecule, which skews CD4+ T cells toward a Th2 response by enhancing their secretion of IL-4.<\/jats:p>","DOI":"10.4049\/jimmunol.169.10.5638","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:04:08Z","timestamp":1398024248000},"page":"5638-5648","source":"Crossref","is-referenced-by-count":66,"title":["Dendritic Cell-Associated Lectin-1: A Novel Dendritic Cell-Associated, C-Type Lectin-Like Molecule Enhances T Cell Secretion of IL-4"],"prefix":"10.1093","volume":"169","author":[{"given":"Elizabeth J","family":"Ryan","sequence":"first","affiliation":[{"name":"Microbiology, University of Washington , Seattle, WA 98195"},{"name":"Regional Primate Research Center, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron J","family":"Marshall","sequence":"additional","affiliation":[{"name":"Microbiology, University of Washington , Seattle, WA 98195"},{"name":"Department of Immunology, University of Manitoba , Winnipeg, Manitoba ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Magaletti","sequence":"additional","affiliation":[{"name":"Regional Primate Research Center, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Floyd","sequence":"additional","affiliation":[{"name":"Microbiology, University of Washington , Seattle, WA 98195"},{"name":"Regional Primate Research Center, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin E","family":"Draves","sequence":"additional","affiliation":[{"name":"Immunology, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N Eric","family":"Olson","sequence":"additional","affiliation":[{"name":"Microbiology, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward A","family":"Clark","sequence":"additional","affiliation":[{"name":"Microbiology, University of Washington , Seattle, WA 98195"},{"name":"Immunology, University of Washington , Seattle, WA 98195"},{"name":"Regional Primate Research Center, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,11,15]]},"reference":[{"key":"2026020209204851700_R1","first-page":"19","volume-title":"Immunol. 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