{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T17:15:36Z","timestamp":1771953336965,"version":"3.50.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Immunization with recombinant heat shock protein 60 (rHsp60) from Histoplasma capsulatum or a region of the protein designated fragment 3 (F3) confers protection from a subsequent challenge in mice. To determine the T cell repertoire involved in the response to Hsp60, T cell clones from C57BL\/6 mice immunized with rHsp60 were generated and examined for V\u03b2 usage by flow cytometry and RT-PCR. V\u03b28.1\/8.2+ T cells were preferentially expanded; other clones bore V\u03b24, -6, or -11. When V\u03b28.1\/8.2+ cells were depleted in mice, V\u03b24+ T cell clones were almost exclusively isolated. Measurement of cytokine production demonstrated that nine of 16 V\u03b28.1\/8.2+ clones were Th1, while only three of 13 non-V\u03b28.1\/8.2+ clones were Th1. In mice immunized with rHsp60, depletion of V\u03b28.1\/8.2+, but not V\u03b26+ plus V\u03b27+, T cells completely abolished the protective efficacy of Hsp60 to lethal and sublethal challenges. Examination of the TCR revealed that a subset of V\u03b28.1\/2+ clones that produced IFN-\u03b3 and were reactive to F3 shared a common CDR3 sequence, DGGQG. Transfer of these T cell clones into TCR \u03b1\/\u03b2\u2212\/\u2212 or IFN-\u03b3\u2212\/\u2212 mice significantly improved survival, while transfer of other V\u03b28.1\/8.2+ clones that were F3 reactive but were Th2 or clones that were not reactive to F3 but were Th1 did not confer protection. These data indicate that a distinct subset of V\u03b28.1\/8.2+ T cells is crucial for the generation of a protective response to rHsp60.<\/jats:p>","DOI":"10.4049\/jimmunol.169.10.5818","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:04:08Z","timestamp":1398024248000},"page":"5818-5826","source":"Crossref","is-referenced-by-count":47,"title":["The Protective Immune Response to Heat Shock Protein 60 of\n                    <i>Histoplasma capsulatum<\/i>\n                    Is Mediated by a Subset of V\u03b28.1\/8.2+ T Cells"],"prefix":"10.1093","volume":"169","author":[{"given":"Mark","family":"Scheckelhoff","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine , Cincinnati, OH 45267"},{"name":"Veterans Affairs Hospital and Division of Infectious Diseases, University of Cincinnati College of Medicine , Cincinnati, OH 45267"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"George S","family":"Deepe","sequence":"additional","affiliation":[{"name":"Veterans Affairs Hospital and Division of Infectious Diseases, University of Cincinnati College of Medicine , Cincinnati, OH 45267"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,11,15]]},"reference":[{"key":"2026020209235340200_R1","first-page":"201","volume-title":"J. 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