{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:08:33Z","timestamp":1772896113881,"version":"3.50.1"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Through the production of cytokines and growth factors the endothelium of secondary lymphoid organs plays a crucial role in controlling lymphocyte migration to the lymphoid microenvironment, an essential step in the initiation of the immune response. Here we demonstrate that direct contact of B cell lines with tonsil-derived human endothelial cells resulted in changes in the phosphorylation state of endothelial cells, causing their functional activation. We found a rapid (&amp;lt;15-s) and transient dephosphorylation, followed by a rapid rephosphorylation of tyrosine residues of the focal adhesion kinase, paxillin, and ERK2. Maximal rephosphorylation occurred after 15\u201330 min of B cell contact. Preincubation of lymphoid B cells with an adhesion-blocking Ab directed against \u03b14\u03b21 integrin abrogated adhesion-mediated changes of endothelial cell tyrosine phosphorylation, suggesting that cell contact was essential. Similar patterns of tyrosine phosphorylation, but with slightly different kinetics were induced after cross-linking of \u03b21 integrin or CD40 on endothelial cells. Functional activation of endothelial cells by B cell adhesion was confirmed by the production of IL-6, IL-8, monocyte chemoattractant protein-1, M-CSF, and macrophage inflammatory protein-1\u03b2 mRNA. However, direct cross-linking of \u03b21 integrin and CD40 failed to accomplish the same functional activation. These data indicate that direct contact of lymphoid B cells with the endothelium from lymphoid tissue induce endothelial cell signaling, resulting in chemokine and cytokine production. This phenomenon may provide a mechanism for the remodeling of the endothelium from lymphoid tissues, thus contributing to the free migration of lymphocytes and other cells into the lymphoid organs.<\/jats:p>","DOI":"10.4049\/jimmunol.169.10.5881","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T00:04:08Z","timestamp":1398038648000},"page":"5881-5888","source":"Crossref","is-referenced-by-count":7,"title":["Adhesion of B Cell Lines to Endothelial Cells from Human Lymphoid Tissue Modulates Tyrosine Phosphorylation and Endothelial Cell Activation"],"prefix":"10.1093","volume":"169","author":[{"given":"Lilian I","family":"Reyes","sequence":"first","affiliation":[{"name":"Departamento de Biologi\u0301a, Facultad de Ciencias, Universidad de Chile and Millennium Institute for Fundamental and Applied Biology , Santiago ,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Escobar","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a, Facultad de Ciencias, Universidad de Chile and Millennium Institute for Fundamental and Applied Biology , Santiago ,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari\u0301a R","family":"Bono","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a, Facultad de Ciencias, Universidad de Chile and Millennium Institute for Fundamental and Applied Biology , Santiago ,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Rosemblatt","sequence":"additional","affiliation":[{"name":"Departamento de Biologi\u0301a, Facultad de Ciencias, Universidad de Chile and Millennium Institute for Fundamental and Applied Biology , Santiago ,","place":["Chile"]},{"name":"Fundacio\u0301n Ciencia para la Vida , Santiago ,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,11,15]]},"reference":[{"key":"2025032711395564000_R1","first-page":"1033","volume-title":"Cell","year":"1991"},{"key":"2025032711395564000_R2","first-page":"827","volume-title":"Annu. 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