{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:15:03Z","timestamp":1791087303146,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>IFN-\u03b3 is critical for the protection against intracellular bacteria through activation of the antimicrobial machinery of phagocytes. Coxiella burnetii, the etiological agent of Q fever, is a strictly intracellular bacterium that inhabits monocytes\/macrophages. We previously showed that IFN-\u03b3 induced C. burnetii killing by promoting the apoptosis of infected monocytes. We show in this study that IFN-\u03b3-induced apoptosis of infected monocytes was characterized by a time- and dose-dependent activation of caspase-3. IFN-\u03b3-mediated caspase-3 activation and C. burnetii killing depend on the expression of membrane TNF. Indeed, TNF was transiently expressed on the cell surface of infected monocytes a few hours after IFN-\u03b3 treatment. In addition, anti-TNF Abs inhibited IFN-\u03b3-mediated caspase-3 activation whereas soluble TNF had no effect on infected cells. Concomitantly, IFN-\u03b3 induced homotypic adherence of C. burnetii-infected monocytes. The latter required the interaction of \u03b22 integrins with CD54. When adherence was disrupted by pipetting, by a combination of Abs specific for CD11b, CD18, and CD54, or by an antisense oligonucleotide targeting CD18 mRNA, both cell apoptosis and bacterial killing induced by IFN-\u03b3 were inhibited. Thus, adherence via CD54\/\u03b22 integrins together with membrane TNF are required to eliminate C. burnetii-infected cells through cell contact-dependent apoptosis. Our results reveal a new component of the antimicrobial arsenal mobilized by IFN-\u03b3 against infection by intracellular bacteria.<\/jats:p>","DOI":"10.4049\/jimmunol.169.11.6309","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T19:41:24Z","timestamp":1398022884000},"page":"6309-6315","source":"Crossref","is-referenced-by-count":50,"title":["IFN-\u03b3-Induced Apoptosis and Microbicidal Activity in Monocytes Harboring the Intracellular Bacterium\n                    <i>Coxiella burnetii<\/i>\n                    Require Membrane TNF and Homotypic Cell Adherence"],"prefix":"10.1093","volume":"169","author":[{"given":"Je\u0301ro\u0302me","family":"Dellacasagrande","sequence":"first","affiliation":[{"name":"Unite\u0301 des Rickettsies, Unite\u0301 Mixte de Recherche 6020, Centre National de la Recherche Scientifique, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e , Marseille ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Ghigo","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, Unite\u0301 Mixte de Recherche 6020, Centre National de la Recherche Scientifique, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e , Marseille ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, Unite\u0301 Mixte de Recherche 6020, Centre National de la Recherche Scientifique, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e , Marseille ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Capo","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, Unite\u0301 Mixte de Recherche 6020, Centre National de la Recherche Scientifique, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e , Marseille ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Louis","family":"Mege","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, Unite\u0301 Mixte de Recherche 6020, Centre National de la Recherche Scientifique, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e , Marseille ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,12,1]]},"reference":[{"key":"2026020209203635100_R1","first-page":"518","volume-title":"Clin. 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