{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:35:24Z","timestamp":1771655724170,"version":"3.50.1"},"reference-count":88,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Phospholipase C\u03b32 (PLC\u03b32) plays a critical role in the functions of the B cell receptor in B cells and of the FcR\u03b3 chain-containing collagen receptor in platelets. Here we report that PLC\u03b32 is also expressed in mast cells and monocytes\/macrophages and is activated by cross-linking of Fc\u03b5R and Fc\u03b3R. Although PLC\u03b32-deficient mice have normal development and numbers of mast cells and monocytes\/macrophages, we demonstrate that PLC\u03b32 is essential for specific functions of Fc\u03b5R and Fc\u03b3R. While PLC\u03b32-deficient mast cells have normal mitogen-activated protein kinase activation and cytokine production at mRNA levels, the mutant cells have impaired Fc\u03b5R-mediated Ca2+ flux and inositol 1,4,5-trisphosphate production, degranulation, and cytokine secretion. As a physiological consequence of the effect of PLC\u03b32 deficiency, the mutant mice are resistant to IgE-mediated cutaneous inflammatory skin reaction. Macrophages from PLC\u03b32-deficient mice have no detectable Fc\u03b3R-mediated Ca2+ flux; however, the mutant cells have normal Fc\u03b3R-mediated phagocytosis. Moreover, PLC\u03b32 plays a nonredundant role in Fc\u03b3R-mediated inflammatory skin reaction.<\/jats:p>","DOI":"10.4049\/jimmunol.169.12.6743","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T00:33:07Z","timestamp":1398040387000},"page":"6743-6752","source":"Crossref","is-referenced-by-count":59,"title":["Phospholipase C\u03b32 Is Essential for Specific Functions of Fc\u03b5R and Fc\u03b3R"],"prefix":"10.1093","volume":"169","author":[{"given":"Renren","family":"Wen","sequence":"first","affiliation":[{"name":"The Blood Research Institute, The Blood Center of Southeastern Wisconsin , Milwaukee, WI 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiann-Tarng","family":"Jou","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, National Taiwan University Hospital , Taipei ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhong","family":"Chen","sequence":"additional","affiliation":[{"name":"The Blood Research Institute, The Blood Center of Southeastern Wisconsin , Milwaukee, WI 53226"},{"name":"Institute of Molecular Medicine, Nanjing University , Nanjing ,","place":["Peoples Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelica","family":"Hoffmeyer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Byk Gulden , Konstanz ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Demin","family":"Wang","sequence":"additional","affiliation":[{"name":"The Blood Research Institute, The Blood Center of Southeastern Wisconsin , Milwaukee, WI 53226"},{"name":"Department of Microbiology and Molecular Genetics, Medical College of Wisconsin , Milwaukee, WI 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,12,15]]},"reference":[{"key":"2025032711492377200_R1","first-page":"121","volume-title":"Curr. 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