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We reported previously that aerosolized Syk antisense oligodeoxynucleotides (ASO) depresses Syk expression in inflammatory cells, the release of mediators from alveolar macrophages, and pulmonary inflammation. To study the effect of Syk ASO in allergic inflammation and airway hyperresponsiveness, we used the Brown Norway rat model of OVA-induced allergic asthma. Syk ASO, delivered in a liposome, carrier\/lipid complex by aerosol to rats, significantly inhibited the Ag-induced inflammatory cell infiltrate in the bronchoalveolar space, decreasing both neutrophilia and eosinophilia. The number of eosinophils in the lung parenchyma was also diminished. Syk ASO also depressed up-regulation of the expression of \u03b22 integrins, \u03b14 integrin, and ICAM-1 in bronchoalveolar lavage leukocytes and reversed the Ag-induced decrease in CD62L expression on neutrophils. Furthermore, the increase in TNF levels in bronchoalveolar lavage following Ag challenge was significantly inhibited. Syk ASO also suppressed Ag-mediated contraction of the trachea in a complementary model. Thus, aerosolized Syk ASO suppresses many of the central components of allergic asthma and inflammation and may provide a new therapeutic approach.<\/jats:p>","DOI":"10.4049\/jimmunol.169.2.1028","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T02:54:25Z","timestamp":1398048865000},"page":"1028-1036","source":"Crossref","is-referenced-by-count":95,"title":["Inhibition of Allergic Inflammation in the Airways Using Aerosolized Antisense to Syk Kinase"],"prefix":"10.1093","volume":"169","author":[{"given":"Grant R","family":"Stenton","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Alberta , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Ulanova","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alberta , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301 E","family":"De\u0301ry","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alberta , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaheed","family":"Merani","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alberta , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moo-Kyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of University of Pennsylvania School of Medicine , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Gilchrist","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alberta , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakshmi","family":"Puttagunta","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, University of Alberta , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorin","family":"Musat-Marcu","sequence":"additional","affiliation":[{"name":"Department of HistoBest Inc. , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"James","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Alberta , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan D","family":"Schreiber","sequence":"additional","affiliation":[{"name":"Department of University of Pennsylvania School of Medicine , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A Dean","family":"Befus","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alberta , Edmonton, Alberta ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,7,15]]},"reference":[{"key":"2025032905530837500_R1","first-page":"1175","volume-title":"Blood","year":"1995"},{"key":"2025032905530837500_R2","first-page":"4209","volume-title":"Mol. 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