{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:51:47Z","timestamp":1793278307088,"version":"4.5.0"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>To define better the molecular basis for follicular dendritic cell (FDC) function, we used PCR-based cDNA subtraction to identify genes specifically expressed in primary FDC isolated from human tonsils. In this work we report the discovery of a novel gene encoding a small secreted protein, which we term FDC-SP (FDC secreted protein). The FDC-SP gene lies on chromosome 4q13 adjacent to clusters of proline-rich salivary peptides and C-X-C chemokines. Human and mouse FDC-SP proteins are structurally unique and contain a conserved N-terminal charged region adjacent to the leader peptide. FDC-SP has a very restricted tissue distribution and is expressed by activated FDCs from tonsils and TNF-\u03b1-activated FDC-like cell lines, but not by B cell lines, primary germinal center B cells, or anti-CD40 plus IL-4-activated B cells. Strikingly, FDC-SP is highly expressed in germinal center light zone, a pattern consistent with expression by FDC. In addition, FDC-SP is expressed in leukocyte-infiltrated tonsil crypts and by LPS- or Staphylococcus aureus Cowan strain 1-activated leukocytes, suggesting that FDC-SP can also be produced in response to innate immunity signals. We provide evidence that FDC-SP is posttranslationally modified and secreted and can bind to the surface of B lymphoma cells, but not T lymphoma cells, consistent with a function as a secreted mediator acting upon B cells. Furthermore, we find that binding of FDC-SP to primary human B cells is markedly enhanced upon activation with the T-dependent activation signals such as anti-CD40 plus IL-4. Together our data identify FDC-SP as a unique secreted peptide with a distinctive expression pattern within the immune system and the ability to specifically bind to activated B cells.<\/jats:p>","DOI":"10.4049\/jimmunol.169.5.2381","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T04:05:51Z","timestamp":1398139551000},"page":"2381-2389","source":"Crossref","is-referenced-by-count":78,"title":["FDC-SP, a Novel Secreted Protein Expressed by Follicular Dendritic Cells"],"prefix":"10.1093","volume":"169","author":[{"given":"Aaron J","family":"Marshall","sequence":"first","affiliation":[{"name":"Department of Immunology, University of Manitoba , Winnipeg ,","place":["Canada"]},{"name":"Department of Microbiology, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quijiang","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Manitoba , Winnipeg ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin E","family":"Draves","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasufumi","family":"Shikishima","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Manitoba , Winnipeg ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kent T","family":"HayGlass","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Manitoba , Winnipeg ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward A","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington , Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,9,1]]},"reference":[{"key":"2025032905541256300_R1","first-page":"2183","volume-title":"Eur. J. Immunol.","year":"1990"},{"key":"2025032905541256300_R2","first-page":"191","volume-title":"Adv. Exp. Med. Biol.","year":"1991"},{"key":"2025032905541256300_R3","first-page":"379","volume-title":"Semin. Immunol.","year":"1991"},{"key":"2025032905541256300_R4","first-page":"2395","volume-title":"Eur. J. Immunol.","year":"1990"},{"key":"2025032905541256300_R5","first-page":"139","volume-title":"Int. Rev. Cytol.","year":"1996"},{"key":"2025032905541256300_R6","first-page":"39","volume-title":"Immunol. Rev.","year":"1997"},{"key":"2025032905541256300_R7","first-page":"181","volume-title":"Immunol. Rev.","year":"2000"},{"key":"2025032905541256300_R8","first-page":"117","volume-title":"Annu. Rev. Immunol.","year":"1994"},{"key":"2025032905541256300_R9","first-page":"91","volume-title":"Annu. Rev. Immunol.","year":"1989"},{"key":"2025032905541256300_R10","first-page":"75","volume-title":"Exp. Mol. Pathol.","year":"1968"},{"key":"2025032905541256300_R11","first-page":"207","volume-title":"Cell. Immunol.","year":"1979"},{"key":"2025032905541256300_R12","first-page":"227","volume-title":"J. Exp. Med.","year":"1968"},{"key":"2025032905541256300_R13","first-page":"643","volume-title":"Immunology","year":"1978"},{"key":"2025032905541256300_R14","first-page":"931","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2025032905541256300_R15","first-page":"855","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2025032905541256300_R16","first-page":"2508","volume-title":"J. Immunol.","year":"2000"},{"key":"2025032905541256300_R17","first-page":"768","volume-title":"J. Immunol.","year":"2000"},{"key":"2025032905541256300_R18","first-page":"462","volume-title":"Nature","year":"1996"},{"key":"2025032905541256300_R19","first-page":"491","volume-title":"Immunity","year":"1997"},{"key":"2025032905541256300_R20","first-page":"2055","volume-title":"J. Exp. Med.","year":"1993"},{"key":"2025032905541256300_R21","first-page":"3345","volume-title":"J. Immunol.","year":"1995"},{"key":"2025032905541256300_R22","first-page":"330","volume-title":"Clin. Exp. Immunol.","year":"1993"},{"key":"2025032905541256300_R23","first-page":"31","volume-title":"J. Immunol.","year":"1993"},{"key":"2025032905541256300_R24","first-page":"3327","volume-title":"J. Immunol.","year":"1992"},{"key":"2025032905541256300_R25","first-page":"545","volume-title":"J. Immunol.","year":"1995"},{"key":"2025032905541256300_R26","first-page":"1101","volume-title":"J. Immunol.","year":"1995"},{"key":"2025032905541256300_R27","first-page":"1173","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2025032905541256300_R28","first-page":"205","volume-title":"Clin. Exp. Immunol.","year":"1986"},{"key":"2025032905541256300_R29","first-page":"27","volume-title":"Cell. Immunol.","year":"1994"},{"key":"2025032905541256300_R30","first-page":"167","volume-title":"J. Clin. Pathol.","year":"1983"},{"key":"2025032905541256300_R31","first-page":"509","volume-title":"Eur. J. Immunol.","year":"1991"},{"key":"2025032905541256300_R32","first-page":"165","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2025032905541256300_R33","first-page":"1077","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2025032905541256300_R34","first-page":"49","volume-title":"J. Immunol.","year":"2001"},{"key":"2025032905541256300_R35","first-page":"799","volume-title":"Nature","year":"1998"},{"key":"2025032905541256300_R36","first-page":"309","volume-title":"Nature","year":"2000"},{"key":"2025032905541256300_R37","first-page":"87","volume-title":"Curr. Top. Microbiol. Immunol.","year":"1999"},{"key":"2025032905541256300_R38","first-page":"159","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2025032905541256300_R39","first-page":"1009","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2025032905541256300_R40","first-page":"403","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2025032905541256300_R41","first-page":"5949","volume-title":"J. Immunol.","year":"1999"},{"key":"2025032905541256300_R42","first-page":"2353","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2025032905541256300_R43","first-page":"533","volume-title":"Acta Pathol. Jpn.","year":"1979"},{"key":"2025032905541256300_R44","first-page":"1072","volume-title":"J. Immunol.","year":"2001"},{"key":"2025032905541256300_R45","first-page":"221","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1996"},{"key":"2025032905541256300_R46","first-page":"1461","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2025032905541256300_R47","first-page":"471","volume-title":"Immunity","year":"2000"},{"key":"2025032905541256300_R48","first-page":"518","volume-title":"Am. J. Kidney Dis.","year":"2001"},{"key":"2025032905541256300_R49","first-page":"705","volume-title":"FASEB J.","year":"1998"},{"key":"2025032905541256300_R50","first-page":"1305","volume-title":"Mol. Cell. 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