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In this work we demonstrate that Cpn infection primes a pathogen-specific CD8+ T cell response in mice. Eighteen H-2b binding peptides representing sequences from 12 Cpn Ags sensitized target cells for MHC class I-restricted lysis by CD8+ CTL generated from the spleens and lungs of infected mice. Peptide-specific IFN-\u03b3-secreting CD8+ T cells were present in local and systemic compartments after primary infection, and these cells expanded after pathogen re-exposure. CD8+ T cell lines to the 18 Cpn epitope-bearing peptides were cytotoxic, displayed a memory phenotype, and secreted IFN-\u03b3 and TNF-\u03b1, but not IL-4. These CTL lines lysed Cpn-infected macrophages, and the lytic activity was inhibited by brefeldin A, indicating endogenous processing of CTL Ags. Finally, Cpn peptide-specific CD8+ CTL suppressed chlamydial growth in vitro by direct lysis of infected cells and by secretion of IFN-\u03b3 and other soluble factors. These studies provide information on the mechanisms by which CD8+ CTL protect against Cpn, furnish the tools to investigate their possible role in immunopathology, and lay the foundation for future work to develop vaccines against acute and chronic Cpn infections.<\/jats:p>","DOI":"10.4049\/jimmunol.169.5.2524","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T04:05:51Z","timestamp":1398139551000},"page":"2524-2535","source":"Crossref","is-referenced-by-count":41,"title":["Multiple <i>Chlamydia<\/i> \u00a0<i>pneumoniae<\/i> Antigens Prime CD8+ Tc1 Responses That Inhibit Intracellular Growth of This Vacuolar Pathogen"],"prefix":"10.1093","volume":"169","author":[{"given":"Benjamin","family":"Wizel","sequence":"first","affiliation":[{"name":"Center for Pulmonary and Infectious Disease Control, University of Texas Health Center , Tyler, TX 75708"},{"name":"Department of Microbiology and Immunology, University of Texas Health Center , Tyler, TX 75708"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry C","family":"Starcher","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Texas Health Center , Tyler, TX 75708"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buka","family":"Samten","sequence":"additional","affiliation":[{"name":"Center for Pulmonary and Infectious Disease Control, University of Texas Health Center , Tyler, TX 75708"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zissis","family":"Chroneos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Texas Health Center , Tyler, TX 75708"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F","family":"Barnes","sequence":"additional","affiliation":[{"name":"Center for Pulmonary and Infectious Disease Control, University of Texas Health Center , Tyler, TX 75708"},{"name":"Department of Microbiology and Immunology, University of Texas Health Center , Tyler, TX 75708"},{"name":"Department of Medicine, University of Texas Health Center , Tyler, TX 75708"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Dzuris","sequence":"additional","affiliation":[{"name":"Epimmune Corp. , San Diego, CA 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuichiro","family":"Higashimoto","sequence":"additional","affiliation":[{"name":"National Cancer Institute , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ettore","family":"Appella","sequence":"additional","affiliation":[{"name":"National Cancer Institute , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"Epimmune Corp. , San Diego, CA 92121"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,9,1]]},"reference":[{"key":"2025032905540275900_R1","first-page":"S402","volume-title":"J. 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