{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T03:06:25Z","timestamp":1770347185349,"version":"3.49.0"},"reference-count":75,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We previously demonstrated induction and expression of CD62E and CD62P in the lungs of mice primed and then challenged with intratracheal (i.t.) SRBC. The current study examined accumulation of endogenous lymphocytes in the lungs of endothelial E- and P-selectin-deficient (E\u2212P\u2212) mice after i.t. SRBC challenge. Compared with syngeneic wild-type (wt) mice, E\u2212P\u2212 mice showed an 85\u201395% decrease in CD8+ T cells and B cells in the lungs at both early and late time points. In contrast, CD4+ T cell accumulation was reduced by \u223c60% early, but equivalent to wt levels later. Surprisingly, many \u03b3\u03b4 T cells were found in lungs and blood of E\u2212P\u2212 mice but were undetectable in the lungs and blood of wt mice. Absolute numbers of peripheral blood CD4, CD8, and B lymphocytes in E\u2212P\u2212 mice equaled or exceeded the levels in wt mice, particularly after challenge. Trafficking studies using \u03b1\u03b2 T lymphoblasts confirmed that the recruitment of circulating cells after challenge was markedly reduced in E\u2212P\u2212 mice. Furthermore, Ag priming occurred normally in both the selectin-deficient and wt mice, because primed lymphocytes from both groups transferred Ag sensitivity into naive wt mice. Lung production of mRNA for six CC and two CXC chemokines after challenge was equivalent by RT-PCR analysis in wt and E\u2212P\u2212 mice. Therefore, reduced lung accumulation of \u03b1\u03b2 T cells and B cells in E\u2212P\u2212 mice did not result from reduced delivery of circulating lymphocytes to the lungs, unsuccessful Ag priming, or defective pulmonary chemokine production. Selectin-dependent lymphocyte recruitment into the lungs following i.t.-SRBC challenge is subset specific and time dependent.<\/jats:p>","DOI":"10.4049\/jimmunol.169.5.2570","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T04:05:51Z","timestamp":1398139551000},"page":"2570-2579","source":"Crossref","is-referenced-by-count":15,"title":["Subset-Specific Reductions in Lung Lymphocyte Accumulation Following Intratracheal Antigen Challenge in Endothelial Selectin-Deficient Mice"],"prefix":"10.1093","volume":"169","author":[{"given":"Jeffrey L","family":"Curtis","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Health Care System , Ann Arbor, MI 48109"},{"name":"Comprehensive Cancer Center, University of Michigan Health Care System , Ann Arbor, MI 48109"},{"name":"Graduate Program in Immunology, University of Michigan Health Care System , Ann Arbor, MI 48109"},{"name":"Pulmonary and Critical Care Medicine Section, Medical Service, Department of Veterans Affairs Care System , Ann Arbor, MI 48105"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne","family":"Sonstein","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Health Care System , Ann Arbor, MI 48109"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald A","family":"Craig","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan Health Care System , Ann Arbor, MI 48109"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jill C","family":"Todt","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Health Care System , Ann Arbor, MI 48109"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randall N","family":"Knibbs","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan Health Care System , Ann Arbor, MI 48109"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Polak","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Health Care System , Ann Arbor, MI 48109"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel C","family":"Bullard","sequence":"additional","affiliation":[{"name":"Department of Genomics and Pathobiology, University of Alabama , Birmingham, AL 35294"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lloyd M","family":"Stoolman","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Michigan Health Care System , Ann Arbor, MI 48109"},{"name":"Comprehensive Cancer Center, University of Michigan Health Care System , Ann Arbor, MI 48109"},{"name":"Graduate Program in Immunology, University of Michigan Health Care System , Ann Arbor, MI 48109"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,9,1]]},"reference":[{"key":"2025032905540813900_R1","first-page":"822","volume-title":"J. 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