{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:27:28Z","timestamp":1787030848909,"version":"build-2736575974"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The proteasome catalytic \u03b2 subunits LMP2, LMP7, and MECL-1 and two proteasome activator proteins, PA28 \u03b1 and \u03b2, are induced following exposure to IFN-\u03b3 in vitro. Induction of these immunosubunits and the PA28 \u03b1\/\u03b2 hetero-oligomer alters proteasome catalytic functions and specificity and enhances production of certain MHC class I epitopes. We sought to determine whether and to what extent proteasome subunit composition is regulated in vivo and to elucidate the mechanisms of such regulation. We analyzed basal expression levels of these inducible genes in normal, IFN-\u03b3-deficient, and Stat-1-deficient mice. Mice of all three genotypes display constitutive expression of the immunosubunits and PA28, demonstrating that basal expression in vivo is independent of endogenous IFN-\u03b3 production. However, basal expression levels are reduced in Stat-1\u2212\/\u2212 mice, demonstrating a role for Stat-1 independent of IFN-\u03b3 signaling. To demonstrate that IFN-\u03b3 can induce these genes in vivo, mice were infected with Histoplasma capsulatum. Elevated expression of these genes followed the same time course as IFN-\u03b3 expression in infected mice. IFN-\u03b3-deficient mice did not display elevated protein expression following infection, suggesting that other inflammatory cytokines produced in infected mice are unable to influence proteasome expression. Cytokines other than IFN-\u03b3 also failed to influence proteasome gene expression in vitro in cell lines that had no basal expression of LMP2, LMP7, or MECL-1. Thus, both in vitro and in vivo data demonstrate that IFN-\u03b3 is essential for up-regulation, but not constitutive expression, of immunoproteasome subunits in mice.<\/jats:p>","DOI":"10.4049\/jimmunol.169.6.3046","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:59:03Z","timestamp":1398031143000},"page":"3046-3052","source":"Crossref","is-referenced-by-count":71,"title":["Regulation of Immunoproteasome Subunit Expression In Vivo Following Pathogenic Fungal Infection"],"prefix":"10.1093","volume":"169","author":[{"given":"Lance F","family":"Barton","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati , Cincinnati, OH 45267"},{"name":"Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social , Mexico City ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reshma","family":"Rangwala","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"George S","family":"Deepe","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J","family":"Monaco","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati , Cincinnati, OH 45267"},{"name":"Howard Hughes Medical Institute, University of Cincinnati , Cincinnati, OH 45267"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,9,15]]},"reference":[{"key":"2026020209212810300_R1","first-page":"533","volume-title":"Science","year":"1995"},{"key":"2026020209212810300_R2","first-page":"729","volume-title":"Annu. 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