{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T06:51:14Z","timestamp":1776063074452,"version":"3.50.1"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Monocytic cells were stimulated with IgG-OVA equivalence immune complexes, mAb reacting with Fc\u03b3RI, Fc\u03b3RIIA, and Fc\u03b3RIII, LPS, TNF-\u03b1, and the combination of ionomycin and phorbol ester, to address their effects on the expression of the mRNAs encoding for chemokines. Stimulation of monocytes with immune complexes induced a rapid expression of macrophage-inflammatory protein (MIP)-1\u03b1, MIP-1\u03b2, and IL-8 mRNAs. In contrast, RANTES mRNA was already detectable in resting cells and only increased after 16 h of stimulation. A similar pattern was observed following homotypic stimulation of Fc\u03b3R with mAb reacting with Fc\u03b3RI and Fc\u03b3RIIA, but not with a mAb reacting with Fc\u03b3RIII, a subtype of receptor not expressed in THP-1 cells, thus indicating that both Fc\u03b3RI and Fc\u03b3RIIA are involved in the response. The pattern of chemokine induction elicited by LPS and the combination of ionomycin and PMA showed some similarities to those produced by Fc\u03b3R cross-linking, although expression of IFN-\u03b3-inducible protein 10 mRNA was also observed in response to those agonists. The production of MIP-1\u03b1, MIP-1\u03b2, and RANTES proteins encompassing the induction of their mRNAs was confirmed by specific ELISA. Experiments to address the transcription factors involved in the regulation of MIP-1\u03b1 using pharmacological agents and EMSA showed the possible involvement of CCAAT\/enhancer-binding protein \u03b2 sites and ruled out the functional significance of both NF-AT and AP-1 sites.<\/jats:p>","DOI":"10.4049\/jimmunol.169.6.3321","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:59:03Z","timestamp":1398031143000},"page":"3321-3328","source":"Crossref","is-referenced-by-count":59,"title":["Activation of Monocytic Cells Through Fc\u03b3 Receptors Induces the Expression of Macrophage-Inflammatory Protein (MIP)-1\u03b1, MIP-1\u03b2, and RANTES"],"prefix":"10.1093","volume":"169","author":[{"given":"Nieves","family":"Ferna\u0301ndez","sequence":"first","affiliation":[{"name":"Instituto de Biologi\u0301a y Gene\u0301tica Molecular, Consejo Superior de Investigaciones Cienti\u0301ficas , Valladolid ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Renedo","sequence":"additional","affiliation":[{"name":"Instituto de Biologi\u0301a y Gene\u0301tica Molecular, Consejo Superior de Investigaciones Cienti\u0301ficas , Valladolid ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Garci\u0301a-Rodri\u0301guez","sequence":"additional","affiliation":[{"name":"Unidad de Investigacio\u0301n, Hospital Cli\u0301nico Universitario , Valladolid ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariano","family":"Sa\u0301nchez Crespo","sequence":"additional","affiliation":[{"name":"Instituto de Biologi\u0301a y Gene\u0301tica Molecular, Consejo Superior de Investigaciones Cienti\u0301ficas , Valladolid ,","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,9,15]]},"reference":[{"key":"2026020209242582900_R1","first-page":"1095","volume-title":"Science","year":"1994"},{"key":"2026020209242582900_R2","first-page":"553","volume-title":"Cell","year":"1994"},{"key":"2026020209242582900_R3","first-page":"181","volume-title":"Immunity","year":"1996"},{"key":"2026020209242582900_R4","first-page":"3161","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020209242582900_R5","first-page":"2165","volume-title":"Eur. J. Immunol.","year":"1996"},{"key":"2026020209242582900_R6","first-page":"887","volume-title":"J. Immunol.","year":"1997"},{"key":"2026020209242582900_R7","first-page":"2321","volume-title":"J. Immunol.","year":"1999"},{"key":"2026020209242582900_R8","first-page":"547","volume-title":"Int. Immunol.","year":"2000"},{"key":"2026020209242582900_R9","first-page":"4039","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020209242582900_R10","first-page":"3433","volume-title":"J. Immunol.","year":"1997"},{"key":"2026020209242582900_R11","first-page":"1361","volume-title":"Eur. J. Immunol.","year":"2001"},{"key":"2026020209242582900_R12","first-page":"157","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026020209242582900_R13","first-page":"1900","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020209242582900_R14","first-page":"1105","volume-title":"J. Immunol.","year":"1999"},{"key":"2026020209242582900_R15","first-page":"331","volume-title":"Am. J. Pathol.","year":"1999"},{"key":"2026020209242582900_R16","first-page":"4793","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020209242582900_R17","first-page":"1515","volume-title":"J. Immunol.","year":"1995"},{"key":"2026020209242582900_R18","first-page":"2650","volume-title":"J. Immunol.","year":"2000"},{"key":"2026020209242582900_R19","first-page":"1939","volume-title":"J. Exp. Med.","year":"1988"},{"key":"2026020209242582900_R20","first-page":"5276","volume-title":"Mol. Cell. Biol.","year":"1993"},{"key":"2026020209242582900_R21","first-page":"173","volume-title":"Gene","year":"1995"},{"key":"2026020209242582900_R22","first-page":"245","volume-title":"Cell. Immunol.","year":"1991"},{"key":"2026020209242582900_R23","first-page":"3646","volume-title":"Mol. Cell. Biol.","year":"1990"},{"key":"2026020209242582900_R24","first-page":"207","volume-title":"Practical Immunology","year":"1991"},{"key":"2026020209242582900_R25","first-page":"1359","volume-title":"Br. J. Pharmacol.","year":"1999"},{"key":"2026020209242582900_R26","first-page":"383","volume-title":"Eur. J. Immunol.","year":"2002"},{"key":"2026020209242582900_R27","first-page":"1304","volume-title":"J. Immunol.","year":"1990"},{"key":"2026020209242582900_R28","first-page":"6137","volume-title":"Mol. Cell. Biol.","year":"1993"},{"key":"2026020209242582900_R29","first-page":"554","volume-title":"J. Leukocyte Biol.","year":"1994"},{"key":"2026020209242582900_R30","first-page":"2396","volume-title":"J. Biol. Chem.","year":"1997"},{"key":"2026020209242582900_R31","first-page":"87","volume-title":"Biochem. J.","year":"1994"},{"key":"2026020209242582900_R32","first-page":"1139","volume-title":"J. Immunol.","year":"1996"},{"key":"2026020209242582900_R33","first-page":"1095","volume-title":"Biochem. Biophys. Res. Commun.","year":"2001"},{"key":"2026020209242582900_R34","first-page":"577","volume-title":"FASEB J.","year":"2001"},{"key":"2026020209242582900_R35","first-page":"6428","volume-title":"J. Virol.","year":"2001"},{"key":"2026020209242582900_R36","first-page":"316","volume-title":"Nucleic Acids Res.","year":"2000"},{"key":"2026020209242582900_R37","first-page":"753","volume-title":"Mol. Pharmacol.","year":"1999"},{"key":"2026020209242582900_R38","first-page":"547","volume-title":"Br. J. Pharmacol.","year":"2001"},{"key":"2026020209242582900_R39","first-page":"2932","volume-title":"Blood","year":"2001"},{"key":"2026020209242582900_R40","first-page":"7104","volume-title":"J. Immunol.","year":"2001"},{"key":"2026020209242582900_R41","first-page":"1692","volume-title":"Mol. Cell. Biol.","year":"2000"},{"key":"2026020209242582900_R42","first-page":"99","volume-title":"Exp. Hematol.","year":"1995"},{"key":"2026020209242582900_R43","first-page":"2207","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1993"},{"key":"2026020209242582900_R44","first-page":"6798","volume-title":"Eur. J. Biochem.","year":"2000"},{"key":"2026020209242582900_R45","first-page":"6259","volume-title":"J. Biol. Chem.","year":"2000"},{"key":"2026020209242582900_R46","first-page":"2601","volume-title":"J. Immunol.","year":"1993"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/169\/6\/3321\/62558990\/3321.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/169\/6\/3321\/62558990\/3321.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T14:34:34Z","timestamp":1770042874000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/169\/6\/3321\/8027726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9]]},"references-count":46,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2002,9,15]]},"published-print":{"date-parts":[[2002,9,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.169.6.3321","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2002,9]]},"published":{"date-parts":[[2002,9]]}}}