{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T04:56:48Z","timestamp":1773896208807,"version":"3.50.1"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Complement receptor (CR) type 2 (CR2\/CD21) is normally expressed only during the immature and mature stages of B cell development. In association with CD19, CR2 plays an important role in enhancing mature B cell responses to foreign Ag. We used a murine V\u03bb2 promoter\/V\u03bb2\u20134 enhancer minigene to develop transgenic mice that initiate expression of human CR2 (hCR2) during the CD43+CD25\u2212 late pro-B cell stage of development. We found peripheral blood B cell numbers reduced by 60% in mice expressing high levels of hCR2 and by 15% in mice with intermediate receptor expression. Splenic B cell populations were altered with an expansion of marginal zone cells, and basal serum IgG levels as well as T-dependent immune responses were also significantly decreased in transgenic mice. Mice expressing the highest levels of hCR2 demonstrated in the bone marrow a slight increase in B220intCD43+CD25\u2212 B cells in association with a substantial decrease in immature and mature B cells, indicative of a developmental block in the pro-B cell stage. These data demonstrate that stage-specific expression of CR2 is necessary for normal B cell development, as premature receptor expression substantially alters this process. Alterations in B cell development are most likely due to engagement of pre-B cell receptor-mediated or other regulatory pathways by hCR2 in a CD19- and possibly C3 ligand-dependent manner.<\/jats:p>","DOI":"10.4049\/jimmunol.169.7.3526","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T19:18:01Z","timestamp":1398107881000},"page":"3526-3535","source":"Crossref","is-referenced-by-count":26,"title":["Expression of Human Complement Receptor Type 2 (CD21) in Mice During Early B Cell Development Results in a Reduction in Mature B Cells and Hypogammaglobulinemia"],"prefix":"10.1093","volume":"169","author":[{"given":"Kevin J","family":"Marchbank","sequence":"first","affiliation":[{"name":"Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine , Heath Park, Cardiff ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liudmila","family":"Kulik","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Immunology, University of Colorado Health Sciences Center , Denver, CO 80262"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew G","family":"Gipson","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Immunology, University of Colorado Health Sciences Center , Denver, CO 80262"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B Paul","family":"Morgan","sequence":"additional","affiliation":[{"name":"Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine , Heath Park, Cardiff ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V Michael","family":"Holers","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Immunology, University of Colorado Health Sciences Center , Denver, CO 80262"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,10,1]]},"reference":[{"key":"2026020209250773500_R1","first-page":"595","volume-title":"Annu. 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