{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T09:37:54Z","timestamp":1778751474444,"version":"3.51.4"},"reference-count":74,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In addition to their overexpression in cancer cells, most of the tumor-associated Ags are expressed at low but detectable levels in normal tissues. It is not clear whether the repertoire of T cells specific for unmutated tumor Ags is shaped by negative selection during T cell development. The transgenic adenocarcinoma of mouse prostate (TRAMP) model is transgenic for the SV40 large T Ag (Tag) under the control of the rat probasin regulatory elements. Although it has been established that T lymphocytes from TRAMP mice are tolerant to SV40 Tag, the mechanism of the tolerance is largely unknown. To examine whether the T cell clonal deletion is responsible for the tolerance, we crossed the TRAMP mice with mice transgenic for a rearranged TCR specific for SV40 Tag presented by the H-2Kk. Double transgenic TRAMP\/TCR mice showed profound thymic deletion of SV40 Tag-reactive T cells, including a 6- to 10-fold reduction in the total thymocyte numbers and a &amp;gt;50-fold reduction in phenotypically mature T cells. Consistent with this finding, we observed that the SV40 Tag and endogenous mouse probasin genes are expressed at low levels in the thymus. These results demonstrate that clonal deletion is a major mechanism for tolerance to Ags previously regarded as prostate-specific, and provide direct evidence that the T cell repertoire specific for an unmutated tumor Ag can be shaped by clonal deletion in the thymus.<\/jats:p>","DOI":"10.4049\/jimmunol.169.9.4761","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:57:06Z","timestamp":1398027426000},"page":"4761-4769","source":"Crossref","is-referenced-by-count":28,"title":["Clonal Deletion of Simian Virus 40 Large T Antigen-Specific T Cells in the Transgenic Adenocarcinoma of Mouse Prostate Mice: An Important Role for Clonal Deletion in Shaping the Repertoire of T Cells Specific for Antigens Overexpressed in Solid Tumors"],"prefix":"10.1093","volume":"169","author":[{"given":"Xincheng","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Xin","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terrence L","family":"Geiger","sequence":"additional","affiliation":[{"name":"Department of Pathology, St. Jude Children\u2019s Research Hospital , Memphis, TN 38105"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,11,1]]},"reference":[{"key":"2026020210025393400_R1","first-page":"1373","volume-title":"J. 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