{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:21:47Z","timestamp":1791116507451,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dendritic cells (DCs) are potent APCs for naive CD8+ T cells and are being investigated as vaccine delivery vehicles. In this study, we examine the CD8+ T cell response to defined peptides from Listeria monocytogenes (LM), lymphocytic choriomeningitis virus, and murine CMV coated singly and in combination onto mature bone marrow-derived DCs (BMDCs). We show that immunization of mice with 2 \u00d7 105 mature BMDCs coated with multiple MHC class I peptides generates a significant Ag-specific CD8+ T cell response in both the spleen and nonlymphoid organs. This immunization resulted in a peptide-specific hierarchy in the magnitude of CD8+ T cell priming and noncoordinate kinetics in response to different peptide epitopes. Kinetics were not exclusively due to specific characteristics of the MHC class I molecule, and were not altered in an Ag-independent manner by concurrent LM infection. Mice immunized with listeriolysin O 91\u201399-coated BMDCs are protected against high dose challenge with virulent LM. This protection was enhanced by diversifying the memory CD8+ T cell compartment, even in the absence of a large increase in Ag-specific CD8+ memory T cells.<\/jats:p>","DOI":"10.4049\/jimmunol.169.9.4936","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T20:57:06Z","timestamp":1398027426000},"page":"4936-4944","source":"Crossref","is-referenced-by-count":34,"title":["Quantitation of CD8+ T Cell Expansion, Memory, and Protective Immunity After Immunization with Peptide-Coated Dendritic Cells"],"prefix":"10.1093","volume":"169","author":[{"given":"Sara E","family":"Hamilton","sequence":"first","affiliation":[{"name":"Interdisciplinary Program in Immunology, University of Iowa , Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John T","family":"Harty","sequence":"additional","affiliation":[{"name":"Interdisciplinary Program in Immunology, University of Iowa , Iowa City, IA 52242"},{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,11,1]]},"reference":[{"key":"2026020209252405000_R1","first-page":"465","volume-title":"J. 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