{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T14:59:02Z","timestamp":1780066742445,"version":"3.54.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tumor membrane Ag immobilized on cell size microspheres (large multivalent immunogen (LMI)) was previously shown to augment tumor-specific CTL activity and reduce tumor growth, and a clinical trial examining this approach is in progress. In the current study, LMI treatment has been examined using adoptive transfer of TCR-transgenic CD8 T cells to visualize Ag-specific cells during the response. OT-I T cells specific for H-2Kb\/OVA257\u2013264 were transferred into mice that were then challenged with LMI made by immobilizing H-2Kb\/OVA257\u2013264 on microspheres (Kb\/OVA257\u2013264-LMI) alone, or along with i.p. challenge with OVA-expressing E.G7 tumor. Kb\/OVA257\u2013264-LMI caused significant reduction of tumor growth when administered to E.G7-bearing mice. When administered alone, the Kb\/OVA257\u2013264-LMI caused only weak clonal expansion of OT-I cells in the spleen and lymph nodes, although most of the OT-I cells up-regulated expression of CD44 and VLA-4. In contrast, Kb\/OVA257\u2013264-LMI administration to E.G7-bearing mice stimulated no detectable expansion of OT-I cells in the spleen and lymph nodes but caused a rapid increase in the number of OT-I cells in the peritoneal cavity, the site of the growing tumor. These results demonstrate the potential for using class I\/tumor peptide complexes for immunotherapy. In addition, they suggest a model for the mechanism of CTL augmentation in which recognition of the LMI Ag results in altered trafficking of the tumor-specific CD8 T cells so that they reach the site of a growing tumor more rapidly and in greater numbers, where they may further expand and acquire effector function.<\/jats:p>","DOI":"10.4049\/jimmunol.170.1.228","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T00:11:17Z","timestamp":1398125477000},"page":"228-235","source":"Crossref","is-referenced-by-count":46,"title":["In Vivo Augmentation of Tumor-Specific CTL Responses by Class I\/Peptide Antigen Complexes on Microspheres (Large Multivalent Immunogen)"],"prefix":"10.1093","volume":"170","author":[{"given":"Jodi","family":"Goldberg","sequence":"first","affiliation":[{"name":"Center for Immunology, Department of Laboratory Medicine and Pathology, University of Minnesota , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Protul","family":"Shrikant","sequence":"additional","affiliation":[{"name":"Center for Immunology, Department of Laboratory Medicine and Pathology, University of Minnesota , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew F","family":"Mescher","sequence":"additional","affiliation":[{"name":"Center for Immunology, Department of Laboratory Medicine and Pathology, University of Minnesota , Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,1,1]]},"reference":[{"key":"2026020210112109500_R1","first-page":"337","volume-title":"Annu. 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