{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:11:32Z","timestamp":1790413892440,"version":"4.1.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mast cells and basophils involved in allergic responses do not have clonotypic Ag receptors. However, they can acquire Ag specificity through binding of Ag-specific IgE to Fc\u03b5RI expressed on their surface. Previous studies demonstrated that IgE binding induced the stabilization and accumulation of Fc\u03b5RI on the cell surface and resulted in up-regulation of Fc\u03b5RI. In this study we have further analyzed the maintenance of IgE-mediated memory in mast cells and basophils in vivo by comparing kinetics of serum IgE levels, Fc\u03b5RI expression, and ability to induce systemic anaphylaxis. A single i.v. injection of trinitrophenyl-specific IgE induced 8-fold up-regulation of Fc\u03b5RI expression on peritoneal mast cells in B cell-deficient (\u03bcm\u2212\/\u2212) mice. Serum IgE levels became undetectable by day 6, but the treatment of mice with anti-IgE mAb induced a significant drop in body temperature on days 14, 28, and 42. The administration of trinitrophenyl -BSA, but not BSA, in place of anti-IgE mAb gave similar results, indicating the Ag specificity of the allergic response. This long term maintenance of Ag-specific reactivity in the allergic response was also observed in normal mice passively sensitized with IgE even though the duration was shorter than that in B cell-deficient mice. The appearance of IgE with a different specificity did not interfere with the maintenance of IgE-mediated memory of mast cells and basophils. These results suggest that IgE-mediated stabilization and up-regulation of Fc\u03b5RI enables mast cells and basophils not only to acquire Ag specificity, but also to maintain memory in vivo for lengthy periods of time.<\/jats:p>","DOI":"10.4049\/jimmunol.170.2.775","type":"journal-article","created":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T00:04:08Z","timestamp":1398038648000},"page":"775-780","source":"Crossref","is-referenced-by-count":74,"title":["Long Term Maintenance of IgE-Mediated Memory in Mast Cells in the Absence of Detectable Serum IgE"],"prefix":"10.1093","volume":"170","author":[{"given":"Shuichi","family":"Kubo","sequence":"first","affiliation":[{"name":"Department of Laboratory Animal Science, Tokyo Metropolitan Organization for Medical Science, Tokyo Metropolitan Institute of Medical Science , Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshinori","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Graduate School of Medicine, Chiba University , Chiba ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunie","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Science, Tokyo Metropolitan Organization for Medical Science, Tokyo Metropolitan Institute of Medical Science , Tokyo ,","place":["Japan"]},{"name":"Japan Society for the Promotion of Science , Saitama ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromichi","family":"Yonekawa","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Science, Tokyo Metropolitan Organization for Medical Science, Tokyo Metropolitan Institute of Medical Science , Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Karasuyama","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Science, Tokyo Metropolitan Organization for Medical Science, Tokyo Metropolitan Institute of Medical Science , Tokyo ,","place":["Japan"]},{"name":"Department of Immune Regulation, Tokyo Medical and Dental University, Graduate School , Tokyo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,1,15]]},"reference":[{"key":"2025032709204804900_R1","first-page":"315","volume-title":"Cell","year":"1994"},{"key":"2025032709204804900_R2","first-page":"333","volume-title":"Annu. 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