{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:35:48Z","timestamp":1791329748071,"version":"4.3.1"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2003,3,1]],"date-time":"2003-03-01T00:00:00Z","timestamp":1046476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The generation and maintenance of virus-specific CD4+ T cells in humans are not well understood. We used short in vitro stimulation assays followed by intracellular cytokine staining to characterize the timing, magnitude, and Ag specificity of CD4+ T cells over the course of primary EBV infection. Lytic and latent protein-specific CD4+ T cells were readily detected at presentation with acute infectious mononucleosis and declined rapidly thereafter. Responses to BZLF-1, BMLF-1, and Epstein-Barr nuclear Ag-3A were more commonly detected than responses to Epstein-Barr nuclear Ag-1. Concurrent analyses of BZLF-1-specific CD4+ and CD8+ T cells revealed differences in the expansion, specificity, and stability of CD4+ and CD8+ T cell-mediated responses over time. Peripheral blood EBV load directly correlated with the frequency of EBV-specific CD4+ T cell responses at presentation and over time, suggesting that EBV-specific CD4+ T cell responses are Ag-driven.<\/jats:p>","DOI":"10.4049\/jimmunol.170.5.2590","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T21:32:44Z","timestamp":1398029564000},"page":"2590-2598","source":"Crossref","is-referenced-by-count":80,"title":["Differential Kinetics and Specificity of EBV-Specific CD4+ and CD8+ T Cells During Primary Infection"],"prefix":"10.1093","volume":"170","author":[{"given":"Melissa L","family":"Precopio","sequence":"first","affiliation":[{"name":"Graduate Program in Immunology\/Virology, University of Massachusetts Medical School , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John L","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology\/Virology, University of Massachusetts Medical School , Worcester, MA 01605"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School , Worcester, MA 01605"},{"name":"Department of Pediatrics, University of Massachusetts Medical School , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney","family":"Willard","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology\/Virology, University of Massachusetts Medical School , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohan","family":"Somasundaran","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Massachusetts Medical School , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Luzuriaga","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology\/Virology, University of Massachusetts Medical School , Worcester, MA 01605"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School , Worcester, MA 01605"},{"name":"Department of Pediatrics, University of Massachusetts Medical School , Worcester, MA 01605"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,3,1]]},"reference":[{"key":"2026020210111273200_R1","first-page":"553","volume-title":"J. 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