{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:41:10Z","timestamp":1770219670349,"version":"3.49.0"},"reference-count":90,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Immunotherapeutic approaches to cancer should focus on novel undertakings that modulate immune responses by synergistic enhancement of antitumor immunological parameters. Cancer vaccines should preferably be composed of multiple defined tumor Ag-specific B and T cell epitopes. To develop a multiepitope vaccine, 12 high ranking B cell epitopes were identified from the extracellular domain of the human epidermal growth factor receptor-2 (HER-2) oncoprotein by computer-aided analysis. Four novel HER-2 B cell epitopes were synthesized as chimeras with a promiscuous T cell epitope (aa 288\u2013302) from the measles virus fusion protein (MVF). Two chimeric peptide vaccines, MVF HER-2316\u2013339 and MVF HER-2485\u2013503 induced high levels of Abs in outbred rabbits, which inhibited tumor cell growth. In addition, Abs induced by a combination of two vaccines, MVF HER-2316\u2013339 and MVF HER-2628\u2013647 down-modulated receptor expression and activated IFN-\u03b3 release better than the individual vaccines. Furthermore, this multiepitope vaccine in combination with IL-12 caused a significant reduction (p = 0.004) in the number of pulmonary metastases induced by challenge with syngeneic tumor cells overexpressing HER-2. Peptide Abs targeting specific sites in the extracellular domain may be used for exploring the oncoprotein\u2019s functions. The multiepitope vaccine may have potential application in the treatment of HER-2-associated cancers.<\/jats:p>","DOI":"10.4049\/jimmunol.170.8.4242","type":"journal-article","created":{"date-parts":[[2014,4,20]],"date-time":"2014-04-20T22:24:43Z","timestamp":1398032683000},"page":"4242-4253","source":"Crossref","is-referenced-by-count":66,"title":["A Chimeric Multi-Human Epidermal Growth Factor Receptor-2 B Cell Epitope Peptide Vaccine Mediates Superior Antitumor Responses"],"prefix":"10.1093","volume":"170","author":[{"given":"Naveen K","family":"Dakappagari","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Pyles","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Parihar","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Immunology, Genetics, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William E","family":"Carson","sequence":"additional","affiliation":[{"name":"Department of Surgery, Ohio State University , Columbus, OH 43210"},{"name":"Department of Arthur G. James Comprehensive Cancer Center, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donn C","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Ohio State University , Columbus, OH 43210"},{"name":"Department of Arthur G. James Comprehensive Cancer Center, Ohio State University , Columbus, OH 43210"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pravin T P","family":"Kaumaya","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Ohio State University , Columbus, OH 43210"},{"name":"Department of Microbiology, Ohio State University , Columbus, OH 43210"},{"name":"Department of Molecular Virology, Immunology, Genetics, Ohio State University , Columbus, OH 43210"},{"name":"Department of Arthur G. 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