{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:54:00Z","timestamp":1790704440177,"version":"4.1.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Characterization of Ag-specific BCR repertoires is essential for understanding disease mechanisms involving humoral immunity. This is optimally done by interrogation of paired H chain V region (VH) and L chain V region (VL) sequences of individual and Ag-specific B cells. By applying single-cell high-throughput sequencing on gut lesion plasma cells (PCs), we have analyzed the transglutaminase 2 (TG2)-specific VH:VL autoantibody repertoire of celiac disease (CD) patients. Autoantibodies against TG2 are a hallmark of CD, and anti-TG2 IgA-producing gut PCs accumulate in patients upon gluten ingestion. Altogether, we analyzed paired VH and VL sequences of 1482 TG2-specific and 1421 non\u2013TG2-specific gut PCs from 10 CD patients. Among TG2-specific PCs, we observed a striking bias in IGHV and IGKV\/IGLV gene usage, as well as pairing preferences with a particular presence of the IGHV5-51:IGKV1-5 pair. Selective and biased VH:VL pairing was particularly evident among expanded clones. In general, TG2-specific PCs had lower numbers of mutations both in VH and VL genes than in non\u2013TG2-specific PCs. TG2-specific PCs using IGHV5-51 had particularly few mutations. Importantly, VL segments paired with IGHV5-51 displayed proportionally low mutation numbers, suggesting that the low mutation rate among IGHV5-51 PCs is dictated by the BCR specificity. Finally, we observed selective amino acid changes in VH and VL and striking CDR3 length and J segment selection among TG2-specific IGHV5-51:IGKV1-5 pairs. Hence this study reveals features of a disease- and Ag-specific autoantibody repertoire with preferred VH:VL usage and pairings, limited mutations, clonal dominance, and selection of particular CDR3 sequences.<\/jats:p>","DOI":"10.4049\/jimmunol.1700169","type":"journal-article","created":{"date-parts":[[2017,6,10]],"date-time":"2017-06-10T01:02:32Z","timestamp":1497056552000},"page":"782-791","source":"Crossref","is-referenced-by-count":63,"title":["High-Throughput Single-Cell Analysis of B Cell Receptor Usage among Autoantigen-Specific Plasma Cells in Celiac Disease"],"prefix":"10.1093","volume":"199","author":[{"given":"Bishnudeo","family":"Roy","sequence":"first","affiliation":[{"name":"Department of Immunology, Centre for Immune Regulation, University of Oslo, Oslo University Hospital , 0372 Oslo,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf S","family":"Neumann","sequence":"additional","affiliation":[{"name":"Department of Immunology, KG Jebsen Coeliac Disease Research Centre, University of Oslo, Oslo University Hospital , 0372 Oslo,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omri","family":"Snir","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre for Immune Regulation, University of Oslo, Oslo University Hospital , 0372 Oslo,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasmus","family":"Iversen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre for Immune Regulation, University of Oslo, Oslo University Hospital , 0372 Oslo,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4959-1409","authenticated-orcid":false,"given":"Geir Kjetil","family":"Sandve","sequence":"additional","affiliation":[{"name":"Department of Informatics, KG Jebsen Coeliac Disease Research Centre, University of Oslo , 0373 Oslo,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Knut E A","family":"Lundin","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, KG Jebsen Coeliac Disease Research Centre, Oslo University Hospital-Rikshospitalet , 0372 Oslo,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8860-704X","authenticated-orcid":false,"given":"Ludvig M","family":"Sollid","sequence":"additional","affiliation":[{"name":"Department of Immunology, Centre for Immune Regulation, University of Oslo, Oslo University Hospital , 0372 Oslo,","place":["Norway"]},{"name":"Department of Immunology, KG Jebsen Coeliac Disease Research Centre, University of Oslo, Oslo University Hospital , 0372 Oslo,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,7,15]]},"reference":[{"key":"2025030614175985500_r1","doi-asserted-by":"crossref","first-page":"4110","DOI":"10.1182\/blood-2012-09-459289","article-title":"A functional BCR in human IgA and IgM plasma cells","volume":"121","author":"Pinto","year":"2013","journal-title":"Blood"},{"key":"2025030614175985500_r2","doi-asserted-by":"crossref","first-page":"5377","DOI":"10.4049\/jimmunol.1001587","article-title":"Rapid generation of rotavirus-specific human monoclonal antibodies from small-intestinal mucosa","volume":"185","author":"Di Niro","year":"2010","journal-title":"J. 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