{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T05:39:12Z","timestamp":1783402752030,"version":"3.54.6"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Synovitis is a key contributor to the inflammatory environment in osteoarthritis (OA) joints. Currently, the biological therapy of OA is not satisfactory in multiple single-target trials on anti-TNF agents, or IL-1 antagonists. Systems biological understanding of the phosphorylation state in OA synovium is warranted to direct further therapeutic strategies. Therefore, in this study, we compared the human synovial phosphoproteome of the OA with the acute joint fracture subjects. We found that OA synovium had significantly more phosphoproteins, and 82 phosphoproteins could only be specifically found in all the OA samples. Differentially expressed proteins of the OA synovium were focusing on endoplasmic reticulum\u2013\/Golgi-associated secretion and negative regulation of cell proliferation, which was verified through an IL-1\u03b2\u2013treated human synoviocyte (HS) in vitro model. With data-independent acquisition\u2013based mass spectrometry, we found that IL-1\u03b2 could induce HS to secrete proteins that were significantly associated with the endosomal\/vacuolar pathway, endoplasmic reticulum\/Golgi secretion, complement activation, and collagen degradation. Especially, we found that while specifically suppressing HS endocytosis, IL-1\u03b2 could activate the secretion of 25 TNF-associated proteins, and the change of SERPINE2 and COL3A1 secretion was verified by immunoblotting. In conclusion, our results suggest that OA synovium has a polarized phosphoproteome to inhibit proliferation and maintain active secretion of HS, whereas IL-1\u03b2 alone can transform HS to produce a synovitis-associated secretome, containing numerous TNF-associated secretory proteins in a TNF-independent mode.<\/jats:p>","DOI":"10.4049\/jimmunol.1700480","type":"journal-article","created":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T15:33:10Z","timestamp":1512142390000},"page":"821-833","source":"Crossref","is-referenced-by-count":12,"title":["Deep Coverage Tissue and Cellular Proteomics Revealed IL-1\u03b2 Can Independently Induce the Secretion of TNF-Associated Proteins from Human Synoviocytes"],"prefix":"10.1093","volume":"200","author":[{"given":"Shengquan","family":"Tang","sequence":"first","affiliation":[{"name":"Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 510632,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyuan","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 510632,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 510632,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 510632,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 510632,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhi","family":"Cui","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 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Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 510632,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7981-8224","authenticated-orcid":false,"given":"Shan","family":"Shen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Jinan University , Guangzhou 510632,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5980-3380","authenticated-orcid":false,"given":"Tong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 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