{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:50:28Z","timestamp":1789469428997,"version":"build-2803163510"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Sarcoidosis is a granulomatous disease that primarily affects the lungs and is characterized by an accumulation of CD4+ T cells in the bronchoalveolar lavage (BAL). Previous work has indicated that HLA-DRB1*03:01+ (DR3+) patients diagnosed with the acute form of the disease, L\u00f6fgren\u2019s syndrome (LS), have an accumulation of CD4+ T cells bearing TCRs using TRAV12-1 (formerly AV2S3). However, the importance of these \u03b1-chains in disease pathogenesis and the paired TCR\u03b2-chain remains unknown. This study aimed to identify expanded \u03b1\u03b2TCR pairs expressed on CD4+ T cells derived from the BAL of DR3+ LS patients. Using a deep-sequencing approach, we determined TCR\u03b1- and TCR\u03b2-chain usage, as well as \u03b1\u03b2TCR pairs expressed on BAL CD4+ T cells from LS patients. TRAV12-1 and TRBV2 (formerly BV22) were the most expanded V region gene segments in DR3+ LS patients relative to control subjects, and TRAV12-1 and TRBV2 CDR3 motifs were shared among multiple DR3+ LS patients. When assessing \u03b1\u03b2TCR pairing, TRAV12-1 preferentially paired with TRBV2, and these TRAV12-1\/TRBV2 TCRs displayed CDR3 homology. These findings suggest that public CD4+ TCR repertoires exist among LS patients and that these T cells are recognizing the putative sarcoidosis-associated Ag(s) in the context of DR3.<\/jats:p>","DOI":"10.4049\/jimmunol.1700570","type":"journal-article","created":{"date-parts":[[2017,8,22]],"date-time":"2017-08-22T00:30:30Z","timestamp":1503361830000},"page":"2279-2290","source":"Crossref","is-referenced-by-count":22,"title":["Shared \u03b1\u03b2 TCR Usage in Lungs of Sarcoidosis Patients with L\u00f6fgren\u2019s Syndrome"],"prefix":"10.1093","volume":"199","author":[{"given":"Angela M","family":"Mitchell","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"},{"name":"Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2946-8718","authenticated-orcid":false,"given":"Ylva","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Department of Medicine, Solna, Center for Molecular Medicine, Karolinska Institutet , SE-171 76 Stockholm,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0835-6904","authenticated-orcid":false,"given":"Michael T","family":"Falta","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J","family":"Munson","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie G","family":"Landry","sequence":"additional","affiliation":[{"name":"Barbara Davis Center for Childhood Diabetes, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Eklund","sequence":"additional","affiliation":[{"name":"Department of Medicine, Solna, Center for Molecular Medicine, Karolinska Institutet , SE-171 76 Stockholm,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"},{"name":"Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"},{"name":"Barbara Davis Center for Childhood Diabetes, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill E","family":"Slansky","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Grunewald","sequence":"additional","affiliation":[{"name":"Department of Medicine, Solna, Center for Molecular Medicine, Karolinska Institutet , SE-171 76 Stockholm,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P","family":"Fontenot","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"},{"name":"Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus , Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,10,1]]},"reference":[{"key":"2025030614465725500_r1","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1056\/NEJM199704243361706","article-title":"Sarcoidosis","volume":"336","author":"Newman","year":"1997","journal-title":"N. 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