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In this study, we provide further evidence of the discordance between ex vivo\u2013isolated and in vitro\u2013cultured CD11c+ cells by analyzing an additional phenotype, the ability to support cytosolic growth of the facultative intracellular bacterial pathogen Listeria monocytogenes. Two days after foodborne infection of mice with GFP-expressing L. monocytogenes, a small percentage of CD103neg and CD103+ conventional dendritic cells (cDC) in the intestinal lamina propria and mesenteric lymph nodes were GFP+. However, in vitro infection of the same subsets of cells harvested from naive mice resulted in inefficient invasion by the bacteria (&amp;lt;0.1% of the inoculum). The few intracellular bacteria detected survived for only a few hours. In contrast, cultured CD103negCD11c+ cells induced by GM-CSF readily supported exponential growth of L. monocytogenes. Flt3 ligand\u2013induced cultures yielded CD103+CD11c+ cells that more closely resembled cDC, with only a modest level of L. monocytogenes replication. For both culture protocols, the longer the cells were maintained in vitro, the more readily they supported intracellular growth. The results of this study suggest that cDC are not a niche for intracellular growth of L. monocytogenes during intestinal infection of mice.<\/jats:p>","DOI":"10.4049\/jimmunol.1700970","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T15:34:49Z","timestamp":1508772889000},"page":"3789-3797","source":"Crossref","is-referenced-by-count":13,"title":["<i>Listeria monocytogenes<\/i>Replicate in Bone Marrow\u2013Derived CD11c+ Cells but Not in Dendritic Cells Isolated from the Murine Gastrointestinal Tract"],"prefix":"10.1093","volume":"199","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7945-8038","authenticated-orcid":false,"given":"Grant S","family":"Jones","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria C","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0716-3518","authenticated-orcid":false,"given":"Sarah E F","family":"D\u2019Orazio","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine , Lexington, KY 40536"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,12,1]]},"reference":[{"key":"2025030614533243100_r1","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.immuni.2006.07.017","article-title":"CD8\u03b1+ dendritic cells are required for efficient entry of Listeria monocytogenes into the spleen","volume":"25","author":"Neuenhahn","year":"2006","journal-title":"Immunity"},{"key":"2025030614533243100_r2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.cellimm.2011.03.001","article-title":"Distinct responses of splenic dendritic cell subsets to infection with Listeria monocytogenes: maturation phenotype, level of infection, and T cell priming capacity ex vivo","volume":"268","author":"Mitchell","year":"2011","journal-title":"Cell. 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