{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:49:34Z","timestamp":1790617774969,"version":"4.1.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Neutrophils are an important source of IL-1\u03b2 secretion in bacterial infections, where they infiltrate affected tissues in log-fold higher numbers than macrophages. Neutrophils also have functional NLRP3 and NLRC4 inflammasomes that can process pro\u2013IL-1\u03b2 to the bioactive 17-kDa form. In the current study, we examined the role of IL-1\u03b2 in response to corneal infection with the filamentous fungus Aspergillus fumigatus and found that neutrophils were the predominant source of bioactive IL-1\u03b2 in the cornea. We also observed that caspase-11\u2212\/\u2212 mice exhibit the same susceptibility phenotype as IL-1\u03b2\u2212\/\u2212, ASC\u2212\/\u2212, NLRP3\u2212\/\u2212, and caspase-1\u2212\/\u2212 mice, with impaired neutrophil recruitment to infected corneas and increased hyphal growth. We further demonstrate that caspase-11 is required for caspase-1 activation and IL-1\u03b2 processing during infection. In vitro, we show that caspase-11 is regulated by the common type I IFN receptor (IFNAR) through JAK-STAT signaling and that caspase-11 is required for speck formation and caspase-1 activity. Aspergillus spores (conidia) stimulate IL-1\u03b2 processing and secretion in neutrophils activation of Dectin-1 and signaling through the Raf1 kinase\/MEKK rather than the spleen tyrosine kinase pathway. Collectively, these findings reveal unexpected regulation of IL-1\u03b2 production by neutrophils in response to pathogenic fungi.<\/jats:p>","DOI":"10.4049\/jimmunol.1701195","type":"journal-article","created":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T14:18:11Z","timestamp":1538144291000},"page":"2767-2775","source":"Crossref","is-referenced-by-count":49,"title":["Neutrophil Caspase-11 Is Required for Cleavage of Caspase-1 and Secretion of IL-1\u03b2 in <i>Aspergillus fumigatus<\/i> Infection"],"prefix":"10.1093","volume":"201","author":[{"given":"Yan","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Case Western Reserve University , Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Abbondante","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of California , Irvine, Irvine, CA 92697"},{"name":"Department of Physiology and Biophysics, University of California , Irvine, Irvine, CA 92697"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mausita","family":"Karmakar","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Case Western Reserve University , Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"de Jesus Carrion","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Case Western Reserve University , Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9270-0693","authenticated-orcid":false,"given":"Chengye","family":"Che","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Case Western Reserve University , Cleveland, OH 44106"},{"name":"Department of Ophthalmology, The Affiliated Hospital of Qingdao University , Qingdao, Shandong, China 266000"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5075-5792","authenticated-orcid":false,"given":"Amy G","family":"Hise","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Case Western Reserve University , Cleveland, OH 44106"},{"name":"Department of Medicine, Louis Stokes Cleveland Veterans Affairs Medical Center , Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0137-7582","authenticated-orcid":false,"given":"Eric","family":"Pearlman","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of California , Irvine, Irvine, CA 92697"},{"name":"Department of Physiology and Biophysics, University of California , Irvine, Irvine, CA 92697"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,11,1]]},"reference":[{"key":"2025030417574023500_r1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1749-6632.2011.06392.x","article-title":"An evolutionary perspective on C-type lectins in infection and immunity","volume":"1253","author":"van den Berg","year":"2012","journal-title":"Ann. 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